Meares, Claude F. and Bryant, Robert G. and Baldeschwieler, John D. and Shirley, David A. (1969) Study of carbonic anhydrase using perturbed angular correlations of gamma radiation. Proceedings of the National Academy of Sciences of the United States of America, 64 (4). pp. 1155-1161. ISSN 0027-8424. https://resolver.caltech.edu/CaltechAUTHORS:MEApnas69
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Abstract
The angular correlation of the 150-247 kev gamma-ray cascade of (111m)Cd is strongly perturbed when this nucleus is bound to the enzyme carbonic anhydrase. A comparison of the perturbed angular correlation for the apoenzyme with that for native carbonic anhydrase confirms that the (111m)Cd binds at the active region of the enzyme. These results provide good evidence that the perturbed angular correlation reflects the effective molecular rotational correlation time at the metal binding site, and that this radioactive nucleus can be used as a rotational tracer to label biological macromolecules. The qualitative dependence of the perturbed angular correlation of the (111m)Cd cascade on the molecular rotational correlation time at the metal binding site is illustrated using a cadmium-complex solution at various temperatures.
Item Type: | Article | ||||||
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Additional Information: | Copyright © 1969 by the National Academy of Sciences. Communicated by Harden M. McConnell, September 15, 1969. The assistance of Mrs. Winifred Heppler, Mr. George Gabor, Mrs. Penny Fink, and Dr. Lloyd Robinson is gratefully acknowledged. | ||||||
Issue or Number: | 4 | ||||||
Record Number: | CaltechAUTHORS:MEApnas69 | ||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:MEApnas69 | ||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||
ID Code: | 1212 | ||||||
Collection: | CaltechAUTHORS | ||||||
Deposited By: | Tony Diaz | ||||||
Deposited On: | 05 Jan 2006 | ||||||
Last Modified: | 02 Oct 2019 22:41 |
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