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Distinct modes of floor plate induction in the chick embryo

Patten, Iain and Kulesa, Paul and Shen, Michael M. and Fraser, Scott and Placzek, Maryisa (2003) Distinct modes of floor plate induction in the chick embryo. Development, 130 (20). pp. 4809-4821. ISSN 0950-1991. doi:10.1242/dev.00694.

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[img] Video (QuickTime) (Movie1 - Movement of cells from the epiblast of a HH stage 4 chick embryo into the ventral midline of the forming neural tube.) - Supplemental Material
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[img] Video (QuickTime) (Movie2 - Movement of cells from ‘area a’ and Hensen’s node of a HH stage 4 chick embryo into the midline of the embryo.) - Supplemental Material
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To begin to reconcile models of floor plate formation in the vertebrate neural tube, we have performed experiments aimed at understanding the development of the early floor plate in the chick embryo. Using real-time analyses of cell behaviour, we provide evidence that the principal contributor to the early neural midline, the future anterior floor plate, exists as a separate population of floor plate precursor cells in the epiblast of the gastrula stage embryo, and does not share a lineage with axial mesoderm. Analysis of the tissue interactions associated with differentiation of these cells to a floor plate fate reveals a role for the nascent prechordal mesoderm, indicating that more than one inductive event is associated with floor plate formation along the length of the neuraxis. We show that Nr1, a chick nodal homologue, is expressed in the nascent prechordal mesoderm and we provide evidence that Nodal signalling can cooperate with Shh to induce the epiblast precursors to a floor-plate fate. These results indicate that a shared lineage with axial mesoderm cells is not a pre-requisite for floor plate differentiation and suggest parallels between the development of the floor plate in amniote and anamniote embryos.

Item Type:Article
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Fraser, Scott0000-0002-5377-0223
Additional Information:Copyright © 2003 The Company of Biologists Limited. Accepted 19 June 2003. First published online 13 August 2003. We thank Simon Ellis for help in early experiments, Liz Illet for technical help and Stephen Szabo for illustrations. We are grateful to Andrew Furley, Sandrine Soubes and the reviewers for helpful comments on the manuscript. This work was supported by the Medical Research Council of Great Britain, the Wellcome Trust and the Yorkshire Cancer Research Trust (to M.P.), by the NIH (to S.E.F. and M.M.S.) and Computational Molecular Biology at the Burroughs-Wellcome Foundation (to P.K.). Movies available online.
Funding AgencyGrant Number
Medical Research Council (MRC)UNSPECIFIED
Yorkshire Cancer TrustUNSPECIFIED
Burroughs-Wellcome FoundationUNSPECIFIED
Subject Keywords:Floor plate, Prechordal mesoderm, Chick, Nodal, Sonic hedgehog
Issue or Number:20
Record Number:CaltechAUTHORS:PATdev03
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:12144
Deposited By: Archive Administrator
Deposited On:24 Oct 2008 23:57
Last Modified:08 Nov 2021 22:26

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