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Mis-regulating segmentation gene expression in Drosophila

Parkhurst, Susan M. and Ish-Horowicz, David (1991) Mis-regulating segmentation gene expression in Drosophila. Development, 111 (4). pp. 1121-1135. ISSN 0950-1991.

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We have used the hunchback (hb) gap-gene promoter to drive ectopic expression of the pair-rule genes fushi tarazu (ftz), even-skipped (eve) and hairy (h). Unexpectedly, flies transformed with such constructs are viable, despite spatial and temporal mis-regulation of pair-rule expression caused by the fusion genes. We show that fusion gene expression is transcriptionally regulated, such that ectopic expression is suppressed when pattern is established, and present evidence indicating that interstripe hb-ftz expression is repressed by eve. These results are considered in terms of redundant control of pair-rule gene striping. We also discuss the potential dangers of using mis-regulated gene expression to analyse normal function.

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Additional Information:Copyright © 1991 by Company of Biologists. {Accepted 7 January 1991) We thank Diethard Tautz for sharing unpublished information and dealing patiently with our many questions; Suki Parks for suggestions and many helpful discussions. We are grateful to Paul Martin and Barbara Luke for help with and use of the scanning electron microscope. We also thank Helen Durbin, Manfred Frasch, Ulrike Gaul, Kate Hooper, Herbert Jackie, Henry Krause, Diethard Tautz and Michael Wilcox for antibodies, and Peter Gergen, Yash Hiromi, Herbert Jackie, Gary Struhl and Diethard Tautz for fly stocks and DNA clones used in this study. We are very grateful to Karen Downs, Peter Gergen, Yash Hiromi, Howard Lipshitz, Suki Parks and Detlef Weigel for critical reading and comments on the manuscript. We thank our colleagues in the lab (Kate Hooper, Sheena Pinchin, Mark Wainwright, Michael Lardelli, Guy Riddihough and Ilan Davis), in the ICRF Developmental Biology Unit, at Caltech and in the fly groups at Princeton for suggestions and stimulating discussions during the course of this work. S.M.P. was supported by a postdoctoral fellowship from the Helen Hay Whitney Foundation.
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Helen Hay Whitney FoundationUNSPECIFIED
Subject Keywords:segmentation gene expression, embryonic pattern formation, pair-rule genes, genetic redundancy, hunchback, mis-regulation, Drosophila
Issue or Number:4
Record Number:CaltechAUTHORS:PARdev91
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:12150
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Deposited On:25 Oct 2008 05:02
Last Modified:03 Oct 2019 00:25

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