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Plaque formation and isolation of pure lines with poliomyelitis virus

Dulbecco, R. and Vogt, Marguerite (1954) Plaque formation and isolation of pure lines with poliomyelitis virus. Journal of Experimental Medicine, 99 (2). pp. 167-182. ISSN 0022-1007. PMCID PMC2180341. http://resolver.caltech.edu/CaltechAUTHORS:DULjem54b

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Abstract

Plaques have been produced with the three types of poliomyelitis viruses on monolayer tissue cultures of monkey kidney and monkey testis. The number of plaques was proportional to the concentration of the virus. Each plaque originates, therefore, from a single virus particle, defined as the virus unit that is unseparable by dilution. The plaques are due to the specific action of the virus since they are suppressed by type-specific antiserum. Pure virus lines were established by isolating the virus population produced in single plaques. These derived virus lines had the same morphological, serological, and pathogenic properties as the parent strain. High titer virus stocks, with titers up to 7 x 10^8 plaque-forming particles per ml., were obtained.


Item Type:Article
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http://jem.rupress.org/cgi/content/abstract/99/2/167PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2180341/PubMed CentralArticle
Additional Information:Copyright, 1954, by The Rockefeller Institute for Medical Research New York. RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. (Received for publication, June 1, 1953) Grateful acknowledgment is made to Dr. C.F. Pait for numerous discussions and for performing the intracerebral inoculation of the monkeys; to Mrs. Kathy Hayden and to Mrs. Patrida Knuth for their valuable technical assistance. Aided by a grant from The National Foundation for Infantile Paralysis, Inc.
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National Foundation for Infantile ParalysisUNSPECIFIED
PubMed Central ID:PMC2180341
Record Number:CaltechAUTHORS:DULjem54b
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:DULjem54b
Usage Policy:RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode.
ID Code:12238
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:30 Oct 2008 16:55
Last Modified:25 Feb 2019 18:17

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