CaltechAUTHORS
  A Caltech Library Service

A Self-Associating Protein Critical for Chromosome Attachment, Division, and Polar Organization in Caulobacter

Ebersbach, Gitte and Briegel, Ariane and Jensen, Grant J. and Jacobs-Wagner, Christine (2008) A Self-Associating Protein Critical for Chromosome Attachment, Division, and Polar Organization in Caulobacter. Cell, 134 (6). pp. 956-968. ISSN 0092-8674. PMCID PMC2614312. https://resolver.caltech.edu/CaltechAUTHORS:EBEc08

[img] PDF - Accepted Version
See Usage Policy.

4Mb
[img]
Preview
PDF (Supplemental Experimental Procedures, Supplemental References, Five Figures, and Two Tables) - Supplemental Material
See Usage Policy.

2278Kb
[img] Video (QuickTime) (Movie S1. Time-Lapse Microscopy Showing FtsZ-YFP Localization during popZ Overexpression) - Supplemental Material
See Usage Policy.

859Kb
[img] Video (QuickTime) (Movie S2. Time-Lapse Microscopy Showing Movement of ParB•ori in Normal-Sized and Elongated ΔpopZ Cells) - Supplemental Material
See Usage Policy.

107Kb
[img] Video (QuickTime) (Movie S3. Time-Lapse Microscopy Showing Movement of ParB•ori in Normal-Sized and Elongated ΔpopZ Cells) - Supplemental Material
See Usage Policy.

312Kb
[img] Video (QuickTime) (Movie S4. Time-Lapse Microscopy Showing Movement of Internal, but not Polar, ParB•ori in a Filamentous, FtsZ-Depleted Cell) - Supplemental Material
See Usage Policy.

509Kb
[img] Video (QuickTime) (Movie S5. Time-Lapse Microscopy Showing that PopZ Captures and Anchors the ParB•ori Complexes at the Cell Poles) - Supplemental Material
See Usage Policy.

397Kb
[img] Video (QuickTime) (Movie S6. Time-Lapse Microscopy Showing that PopZ Captures and Anchors the ParB•ori Complexes at the Cell Poles) - Supplemental Material
See Usage Policy.

224Kb
[img] Video (QuickTime) (Movie S7. Time-Lapse Microscopy Showing Movement of MipZ-YFP in ΔpopZ Cells) - Supplemental Material
See Usage Policy.

335Kb
[img] Video (QuickTime) (Movie S8. Time-Lapse Microscopy Showing FtsZ-YFP Localization in ΔpopZ Cells) - Supplemental Material
See Usage Policy.

799Kb
[img] Video (QuickTime) (Movie S9. Time-Lapse Microscopy Showing DivJ-YFP Localization in ΔpopZ Cells) - Supplemental Material
See Usage Policy.

318Kb
[img] Video (QuickTime) (Movie S10. Timelapse Microscopy Showing mYFP-PopZ Assembly in E. coli) - Supplemental Material
See Usage Policy.

221Kb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:EBEc08

Abstract

Cell polarization is an integral part of many unrelated bacterial processes. How intrinsic cell polarization is achieved is poorly understood. Here, we provide evidence that Caulobacter crescentus uses a multimeric pole-organizing factor (PopZ) that serves as a hub to concurrently achieve several polarizing functions. During chromosome segregation, polar PopZ captures the ParB•ori complex and thereby anchors sister chromosomes at opposite poles. This step is essential for stabilizing bipolar gradients of a cell division inhibitor and setting up division near midcell. PopZ also affects polar stalk morphogenesis and mediates the polar localization of the morphogenetic and cell cycle signaling proteins CckA and DivJ. Polar accumulation of PopZ, which is central to its polarizing activity, can be achieved independently of division and does not appear to be dictated by the pole curvature. Instead, evidence suggests that localization of PopZ largely relies on PopZ multimerization in chromosome-free regions, consistent with a self-organizing mechanism.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.cell.2008.07.016DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2614312/PubMed CentralArticle
http://www.sciencedirect.com/science/article/pii/S0092867408009343PublisherArticle
http://www.cell.com/cgi/content/full/134/6/956/DC1/PublisherSupplemental Data
ORCID:
AuthorORCID
Briegel, Ariane0000-0003-3733-3725
Jensen, Grant J.0000-0003-1556-4864
Additional Information:© 2008 Elsevier. Received 26 November 2007; revised 18 April 2008; accepted 11 July 2008. Published: September 18, 2008. Available online 18 September 2008. We thank M. Mooseker, E. Dufresne, T. Emonet, and J. Wolenski for valuable discussions; K. Gerdes, J. Gober, A. Newton, N. Ohta, L. Shapiro, M. Thanbichler, and P. Viollier for supplying strains or antibody; P. Angelastro, A. Jackson, H. Lam, and W. Schofield for construction of strains; H. J. Ding and D. Rosenman for computational help; M. Mooseker, Z. Jiang, and G. Charbon for assistance with EM; E. Folta-Stogniew and the Keck Foundation Biotechnology Resource Laboratory at Yale for the biophysical analyses, and the Jacobs-Wagner laboratory and T. Emonet for critical reading of the manuscript. G.E. was supported by postdoctoral fellowships from the Villum Kann Rasmussen Foundation and the Danish Natural Science Research Council. This work was funded in part by National Institutes of Health (GM065835 to C.J.-W. and AI067548 to G.J.J.), gifts to Caltech from the Gordon and Betty Moore Foundation and Agouron Institute (to G.J.J.) and the Pew Scholars Program in the Biological Sciences sponsored by the Pew Charitable trust (to C.J.-W.). C.J.-W. is a Howard Hughes Medical Institute investigator. Supplemental Data include Supplemental Experimental Procedures, Supplemental References, five figures, and two tables and can be found with this article online at http://www.cell.com/cgi/content/full/134/6/956/DC1/.
Funders:
Funding AgencyGrant Number
Villum Kann Rasmussen FoundationUNSPECIFIED
Danish Natural Science Research CouncilUNSPECIFIED
NIHGM065835
NIHAI067548
Gordon and Betty Moore FoundationUNSPECIFIED
Agouron InstituteUNSPECIFIED
Pew Charitable TrustUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:6
PubMed Central ID:PMC2614312
Record Number:CaltechAUTHORS:EBEc08
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:EBEc08
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:12246
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:30 Oct 2008 22:51
Last Modified:03 Oct 2019 00:26

Repository Staff Only: item control page