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Characterization of an Infectivity Assay for the Ribonucleic Acid of Bacteriophage MS2

Strauss, James H., Jr. and Sinsheimer, Robert L. (1967) Characterization of an Infectivity Assay for the Ribonucleic Acid of Bacteriophage MS2. Journal of Virology, 1 (4). pp. 711-716. ISSN 0022-538X. PMCID PMC375345.

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An infectivity assay for MS2 ribonucleic acid (RNA), which uses bacterial spheroplasts of an F– strain of Escherichia coli, has an efficiency of 10^–5 infected spheroplasts per RNA molecule. The characteristics of this assay and the influence of several parameters are presented. Important variables include the duration of exposure of the cells to lysozyme-ethylenediaminetetraacetic acid, the duration of exposure of the spheroplast stock to the RNA solutions before dilution, the concentration of RNA, and the presence of competing RNA. The growth kinetics of the virus in the infected spheroplasts and the extent of lysis have also been studied.

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Additional Information:Copyright © 1967 American Society for Microbiology. Received for publication 24 April 1967. We thank Gloria Davis for competent assistance with some of the infectivity assays. We would also like to thank E.R. Glowacki for her aid and inspiration. This investigation was supported by Public Health Service research grant GM 13554. The results presented here are part of a thesis by James H. Strauss, Jr., submitted to the California Institute of Technology in partial fulfillment of the requirements for the Ph.D. degree.
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Issue or Number:4
PubMed Central ID:PMC375345
Record Number:CaltechAUTHORS:STRAjvir67
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:12362
Deposited By: Archive Administrator
Deposited On:13 Nov 2008 06:08
Last Modified:03 Oct 2019 00:27

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