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Notochord grafts do not suppress formation of neural crest cells or commissural neurons

Artinger, Kristin B. and Bronner-Fraser, Marianne (1992) Notochord grafts do not suppress formation of neural crest cells or commissural neurons. Development, 116 (4). pp. 877-887. ISSN 0950-1991.

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Grafting experiments previously have established that the notochord affects dorsoventral polarity of the neural tube by inducing the formation of ventral structures such as motor neurons and the floor plate. Here, we examine if the notochord inhibits formation of dorsal structures by grafting a notochord within or adjacent to the dorsal neural tube prior to or shortly after tube closure. In all cases, neural crest cells emigrated from the neural tube adjacent to the ectopic notochord. When analyzed at stages after ganglion formation, the dorsal root ganglia appeared reduced in size and shifted in position in embryos receiving grafts. Another dorsal cell type, commissural neurons, identified by CRABP and neurofilament immunoreactivity, differentiated in the vicinity of the ectopic notochord. Numerous neuronal cell bodies and axonal processes were observed within the induced, but not endogenous, floor plate 1 to 2 days after implantation but appeared to be cleared with time. These results suggest that dorsally implanted notochords cannot prevent the formation of neural crest cells or commissural neurons, but can alter the size and position of neural crest-derived dorsal root ganglia.

Item Type:Article
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Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:Copyright © 1992 by Company of Biologists. (Accepted 9 September 1992) We thank Drs Scott Fraser, Mark Selleck, John Sechrist and Zoe Pettway for helpful comments on the manuscript, Dr John Sechrist for helping with analysis of neurofilament immunoreactivity and Mary Flowers for her technical assistance. We gratefully acknowledge the generous gifts of antibodies from a number of colleagues: Drs H. Tanaka and T. Yamada for the SC1 antibody, Dr Virginia Lee for the neurofilament antibody and Drs Ulf Eriksson and Malcolm Maden for the anti-CRABP antibodies. This work was supported by USPHS HD-25138.
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Subject Keywords:neural crest cells, dorsal root ganglion, commissural neurons, ventral roots
Issue or Number:4
Record Number:CaltechAUTHORS:ARTdev92
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:12382
Deposited On:14 Nov 2008 18:41
Last Modified:03 Oct 2019 00:28

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