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Dynamic Analyses of Drosophila Gastrulation Provide Insights into Collective Cell Migration

McMahon, Amy and Supatto, Willy and Fraser, Scott E. and Stathopoulos, Angelike (2008) Dynamic Analyses of Drosophila Gastrulation Provide Insights into Collective Cell Migration. Science, 322 (5907). pp. 1546-1550. ISSN 0036-8075. PMCID PMC2801059. doi:10.1126/science.1167094. https://resolver.caltech.edu/CaltechAUTHORS:MCMsci08

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Abstract

The concerted movement of cells from different germ layers contributes to morphogenesis during early embryonic development. Using an optimized imaging approach and quantitative methods, we analyzed the trajectories of hundreds of ectodermal cells and internalized mesodermal cells within Drosophila embryos over 2 hours during gastrulation. We found a high level of cellular organization, with mesoderm cell movements correlating with some but not all ectoderm movements. During migration, the mesoderm population underwent two ordered waves of cell division and synchronous cell intercalation, and cells at the leading edge stably maintained position. Fibroblast growth factor (FGF) signaling guides mesodermal cell migration; however, we found some directed dorsal migration in an FGF receptor mutant, which suggests that additional signals are involved. Thus, decomposing complex cellular movements can provide detailed insights into collective cell migration.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.1167094DOIArticle
http://www.sciencemag.org/cgi/content/abstract/322/5907/1546PublisherArticle
http://www.sciencemag.org/cgi/content/full/322/5907/1546/DC1PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2801059/PubMed CentralArticle
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5377-0223
Stathopoulos, Angelike0000-0001-6597-2036
Additional Information:© 2008 American Association for the Advancement of Science. 16 May 2008; accepted 12 November 2008. We thank M. Levine and E. Meyerowiz for comments on the manuscript, M. Liebling for advice on Imaris and Matlab, and the Caltech Biological Imaging Center for sharing equipment. This work was supported by grants to A.S. from NIH (R01 GM078542), the Searle Scholars Program, and the March of Dimes (Basil O'Conner Starter Scholar Award, 5-FY06-12), and grants to S.F. from the Caltech Beckman Institute and NIH (Center for Excellence in Genomic Science grant P50 HG004071). Supporting Online Material: http://www.sciencemag.org/cgi/content/full/322/5907/1546/DC1 Materials and Methods; Figs. S1 to S7; Movie S1 to S11.
Funders:
Funding AgencyGrant Number
NIHR01 GM078542
Searle Scholars ProgramUNSPECIFIED
March of Dimes5-FY06-12
Caltech Beckman InstituteUNSPECIFIED
NIHP50 HG004071
Issue or Number:5907
PubMed Central ID:PMC2801059
DOI:10.1126/science.1167094
Record Number:CaltechAUTHORS:MCMsci08
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:MCMsci08
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:12529
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:16 Dec 2008 00:45
Last Modified:08 Nov 2021 22:29

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