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Gene expression profiling of light-induced retinal degeneration in phototransduction gene knockout mice

Krishnan, Jayalakshmi and Chen, Jiayan and Shin, Kum-Joo and Hwang, Jong-Ik and Han, Sang-Uk and Lee, Gwang and Choi, Sangdun (2008) Gene expression profiling of light-induced retinal degeneration in phototransduction gene knockout mice. Experimental and Molecular Medicine, 40 (5). pp. 495-504. ISSN 1226-3613. doi:10.3858/emm.2008.40.5.495.

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Exposure to light can induce photoreceptor cell death and exacerbate retinal degeneration. In this study, mice with genetic knockout of several genes, including rhodopsin kinase (Rhok-/-), arrestin (Sag-/-), transducin (Gnat1-/-), c-Fos (c-Fos-/-) and arrestin/transducin (Sag-/-/Gnat1-/-), were examined. We measured the expression levels of thousands of genes in order to investigate their roles in phototransduction signaling in light-induced retinal degeneration using DNA microarray technology and then further explored the gene network using pathway analysis tools. Several cascades of gene components were induced or inhibited as a result of corresponding gene knockout under specific light conditions. Transducin deletion blocked the apoptotic signaling induced by exposure to low light conditions, and it did not require c-Fos/AP-1. Deletion of c-Fos blocked the apoptotic signaling induced by exposure to high intensity light. In the present study, we identified many gene transcripts that are essential for the initiation of light-induced rod degeneration and proposed several important networks that are involved in pro- and anti-apoptotic signaling. We also demonstrated the different cascades of gene components that participate in apoptotic signaling under specific light conditions.

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Additional Information:Copyright © 2008 Korean Society of Medical Biochemistry and Molecular Biology. Accepted 15 May 2008. This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2006-311-C00482), and it was also partly supported by a Korea Science and Engineering Foundation (KOSEF) grant funded by the Korean government (MOST) (R01-2007-000-20533-0).
Funding AgencyGrant Number
Korea Research FoundationKRF-2006-311-C00482
Korea Science and Engineering FoundationR01-2007-000-20533-0
Subject Keywords:arrestin; gene expression profiling; oligonucleotide array sequence analysis; proto-oncogene proteins c-fos; retinal degeneration; transducin
Issue or Number:5
Record Number:CaltechAUTHORS:KRIemm08
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:12552
Deposited By: Archive Administrator
Deposited On:16 Dec 2008 23:49
Last Modified:08 Nov 2021 22:29

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