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Redmond Red as a Redox Probe for the DNA-Mediated Detection of Abasic Sites

Buzzeo, Marisa C. and Barton, Jacqueline K. (2008) Redmond Red as a Redox Probe for the DNA-Mediated Detection of Abasic Sites. Bioconjugate Chemistry, 19 (11). pp. 2110-2112. ISSN 1043-1802. PMCID PMC2747513. https://resolver.caltech.edu/CaltechAUTHORS:BUZbc08

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Abstract

Redmond Red, a fluoropore containing a redox-active phenoxazine core, has been explored as a new electrochemical probe for the detection of abasic sites in double-stranded DNA. The electrochemical behavior of Redmond Red-modified DNA at gold surfaces exhibits stable, quasi-reversible voltammetry with a midpoint potential centered around −50 mV versus NHE. Importantly, with Redmond Red positioned opposite an abasic site within the DNA duplex, the electrochemical response is significantly enhanced compared to Redmond Red positioned across from a base. Redmond Red, reporting only if well-stacked in the duplex, represents a sensitive probe to detect abasic sites electrochemically in a DNA-mediated reaction.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/bc800339yDOIArticle
http://pubs.acs.org/doi/abs/10.1021/bc800339yPublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/bc800339yPublisherSupporting Information
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2747513/PubMed CentralArticle
ORCID:
AuthorORCID
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:Copyright © 2008 American Chemical Society. Received August 6, 2008; Revised Manuscript Received September 9, 2008. Publication Date (Web): October 3, 2008. We are grateful to the National Institutes of Health (GM 61077) for their financial support.
Funders:
Funding AgencyGrant Number
NIHGM61077
Issue or Number:11
PubMed Central ID:PMC2747513
Record Number:CaltechAUTHORS:BUZbc08
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:BUZbc08
Official Citation:Redmond Red as a Redox Probe for the DNA-Mediated Detection of Abasic Sites Marisa C. Buzzeo and Jacqueline K. Barton Bioconjugate Chemistry 2008 19 (11), 2110-2112 DOI: 10.1021/bc800339y
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:12558
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:13 Dec 2008 00:12
Last Modified:03 Oct 2019 00:29

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