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Effects of multiple-bond ruptures on kinetic parameters extracted from force spectroscopy measurements: Revisiting biotin-streptavidin interactions

Guo, Senli and Ray, Chad and Kirkpatrick, Andrea and Lad, Nimit and Akhremitchev, Boris B. (2008) Effects of multiple-bond ruptures on kinetic parameters extracted from force spectroscopy measurements: Revisiting biotin-streptavidin interactions. Biophysical Journal, 95 (8). pp. 3964-3976. ISSN 0006-3495. https://resolver.caltech.edu/CaltechAUTHORS:GUObj08

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Abstract

Force spectroscopy measurements of the rupture of the molecular bond between biotin and streptavidin often results in a wide distribution of rupture forces. We attribute the long tail of high rupture forces to the nearly simultaneous rupture of more than one molecular bond. To decrease the number of possible bonds, we employed hydrophilic polymeric tethers to attach biotin molecules to the atomic force microscope probe. It is shown that the measured distributions of rupture forces still contain high forces that cannot be described by the forced dissociation from a deep potential well. We employed a recently developed analytical model of simultaneous rupture of two bonds connected by polymer tethers with uneven length to fit the measured distributions. The resulting kinetic parameters agree with the energy landscape predicted by molecular dynamics simulations. It is demonstrated that when more than one molecular bond might rupture during the pulling measurements there is a noise-limited range of probe velocities where the kinetic parameters measured by force spectroscopy correspond to the true energy landscape. Outside this range of velocities, the kinetic parameters extracted by using the standard most probable force approach might be interpreted as artificial energy barriers that are not present in the actual energy landscape. Factors that affect the range of useful velocities are discussed.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1529/biophysj.108.133900DOIArticle
Additional Information:© 2008 Biophysical Society. Submitted March 20, 2008, and accepted for publication June 19, 2008. Originally published as Biophys J. BioFAST on July 11, 2008. The authors thank Duke University and the National Science Foundation (grant CHE-0719043) for financial support.
Funders:
Funding AgencyGrant Number
Duke UniversityUNSPECIFIED
NSFCHE-0719043
Subject Keywords:MOLECULE PULLING EXPERIMENTS; LIGAND-RECEPTOR PAIRS; ENERGY LANDSCAPES; SINGLE MOLECULES; ADHESION BONDS; SYSTEMATIC-ERRORS; BIOLOGICAL BONDS; AVIDIN-BIOTIN; MICROSCOPY; STRENGTH
Issue or Number:8
Record Number:CaltechAUTHORS:GUObj08
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:GUObj08
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:12607
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:05 Feb 2009 22:09
Last Modified:03 Oct 2019 00:30

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