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Microfluidic polymerase chain reaction

Maltezos, George and Gomez, Alvaro and Zhong, Jiang and Gomez, Frank A. and Scherer, Axel (2008) Microfluidic polymerase chain reaction. Applied Physics Letters, 93 (24). Art. No. 243901. ISSN 0003-6951. https://resolver.caltech.edu/CaltechAUTHORS:MALapl08

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Abstract

We implement microfluidic technology to miniaturize a thermal cycling system for amplifying DNA fragments. By using a microfluidic thermal heat exchanger to cool a Peltier junction, we have demonstrated rapid heating and cooling of small volumes of solution. We use a miniature K-type thermocouple to provide a means for in situ sensing of the temperature inside the microrefrigeration system. By combining the thermocouple, two power supplies controlled by a relay system, and computer automation, we reproduce the function of a commercial polymerase chain reaction thermal cycler and demonstrate amplification of a DNA sample of about 1000 base pairs.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1063/1.3046789DOIUNSPECIFIED
http://link.aip.org/link/?APPLAB/93/243901/1PublisherUNSPECIFIED
Additional Information:© 2008 American Institute of Physics. Received 4 July 2008; accepted 12 November 2008; published 16 December 2008. The authors gratefully acknowledge financial support for this research by grants from the National Science Foundation (Grant Nos. CHE-0515363 and DMR-0351848).
Funders:
Funding AgencyGrant Number
National Science FoundationCHE-0515363
National Science FoundationDMR-0351848
Subject Keywords:bioMEMS, DNA, heat exchangers, microfluidics, molecular biophysics, thermocouples
Issue or Number:24
Record Number:CaltechAUTHORS:MALapl08
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:MALapl08
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:12688
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:19 Dec 2008 23:27
Last Modified:03 Oct 2019 00:30

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