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A large-scale analysis of mRNAs expressed by primary mesenchyme cells of the sea urchin embryo

Zhu, Xiaodong and Maharias, Gregory and llies, Michele and Cameron, R. Andrew and Davidson, Eric H. and Ettensohn, Charles A. (2001) A large-scale analysis of mRNAs expressed by primary mesenchyme cells of the sea urchin embryo. Development, 128 (13). pp. 2615-2627. ISSN 0950-1991. https://resolver.caltech.edu/CaltechAUTHORS:ZHUdev01

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Abstract

The primary mesenchyme cells (PMCs) of the sea urchin embryo have been an important model system for the analysis of cell behavior during gastrulation. To gain an improved understanding of the molecular basis of PMC behavior, a set of 8293 expressed sequenced tags (ESTs) was derived from an enriched population of mid-gastrula stage PMCs. These ESTs represented approximately 1200 distinct proteins, or about 15% of the mRNAs expressed by the gastrula stage embryo. 655 proteins were similar (P<10-7 by BLAST comparisons) to other proteins in GenBank, for which some information is available concerning expression and/or function. Another 116 were similar to ESTs identified in other organisms, but not further characterized. We conservatively estimate that sequences encoding at least 435 additional proteins were included in the pool of ESTs that did not yield matches by BLAST analysis. The collection of newly identified proteins includes many candidate regulators of primary mesenchyme morphogenesis, including PMC-specific extracellular matrix proteins, cell surface proteins, spicule matrix proteins and transcription factors. This work provides a basis for linking specific molecular changes to specific cell behaviors during gastrulation. Our analysis has also led to the cloning of several key components of signaling pathways that play crucial roles in early sea urchin development.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dev.biologists.org/cgi/content/abstract/128/13/2615PublisherArticle
ORCID:
AuthorORCID
Cameron, R. Andrew0000-0003-3947-6041
Additional Information:© 2001 The Company of Biologists Limited. Accepted 18 April 2001. This work was supported by NIH Grant HD24690 (C.A.E.), NSF Grant IBN-9817988 (C.A.E.), and by a grant from the Stowers Institute for Medical Research.
Funders:
Funding AgencyGrant Number
NIHHD24690
NSFIBN-9817988
Stowers Institute for Medical ResearchUNSPECIFIED
Subject Keywords:Morphogenesis, Gastrulation, Cell migration, Cell fusion, Sea urchin embryo, Primary mesenchyme cells, EST analysis
Issue or Number:13
Record Number:CaltechAUTHORS:ZHUdev01
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:ZHUdev01
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:12700
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:20 Dec 2008 07:50
Last Modified:03 Oct 2019 00:30

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