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Disruption of an RNA helicase/RNAse III gene in Arabidopsis causes unregulated cell division in floral meristems

Jacobsen, Steven E. and Running, Mark P. and Meyerowitz, Elliot M. (1999) Disruption of an RNA helicase/RNAse III gene in Arabidopsis causes unregulated cell division in floral meristems. Development, 126 (23). pp. 5231-5243. ISSN 0950-1991.

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Arabidopsis thaliana floral meristems are determinate structures that produce a defined number of organs, after which cell division ceases. A new recessive mutant, carpel factory (caf), converts the floral meristems to an indeterminate state. They produce extra whorls of stamens, and an indefinite number of carpels. Thus, CAF appears to suppress cell division in floral meristems. The function of CAF is partially redundant with the function of the CLAVATA (CLV) and SUPERMAN (SUP) genes, as caf clv and caf sup double mutants show dramatically enhanced floral meristem over-proliferation. caf mutant plants also show other defects, including absence of axillary inflorescence meristems, and abnormally shaped leaves and floral organs. The CAF gene was cloned and found to encode a putative protein of 1909 amino acids containing an N-terminal DExH/DEAD-box type RNA helicase domain attached to a C-terminal RNaseIII-like domain. A very similar protein of unknown function is encoded by a fungal and an animal genome. Helicase proteins are involved in a number of processes, including specific mRNA localization and mRNA splicing. RNase III proteins are involved in the processing of rRNA and some mRNA molecules. Thus CAF may act through some type of RNA processing event(s). CAF gives rise to two major transcripts of 2.5 and 6.2 kb. In situ hybridization experiments show that CAF RNA is expressed throughout all shoot tissues.

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Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 1999 Company of Biologists. Accepted 22 September; published on WWW 9 November 1999. The authors thank Ken Feldmann for providing the insertional mutagenesis population from which the caf mutant was isolated, and the Arabidopsis Biological Resource Center for materials. We also thank Leonard Medrano for RFLP mapping the CAF gene, and Pam Green for useful discussions. S.E.J. was supported by an NIH postdoctoral fellowship (GM15964). M.P.R. was a Howard Hughes Predoctoral Fellow. This work was supported by NIH grant GM45697 to E.M.M.
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Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Subject Keywords:Flower development, Meristem determinacy, RNaseIII, Helicase, Arabidopsis thaliana, carpel factory (caf)
Issue or Number:23
Record Number:CaltechAUTHORS:JACdev99
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Official Citation:Disruption of an RNA helicase/RNAse III gene in Arabidopsis causes unregulated cell division in floral meristems S.E. Jacobsen, M.P. Running, E.M. Meyerowitz Development 1999 126: 5231-5243
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:12776
Deposited By: Archive Administrator
Deposited On:23 Dec 2008 05:02
Last Modified:03 Oct 2019 00:31

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