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Differential regulation of transcription factor gene expression and phenotypic markers in developing sympathetic neurons

Groves, Andrew K. and George, Kathleen M. and Tissier-Seta, Jean-Phillipe and Engel, James Douglas and Brunet, Jean-François and Anderson, David J. (1995) Differential regulation of transcription factor gene expression and phenotypic markers in developing sympathetic neurons. Development, 121 (3). pp. 887-901. ISSN 0950-1991.

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We have examined the regulation of transcription factor gene expression and phenotypic markers in developing chick sympathetic neurons. Sympathetic progenitor cells first express the bHLH transcriptional regulator Cash-1 (a chicken achaete-scute homologue), followed by coordinate expression of Phox2, a paired homeodomain protein, and GATA-2, a zinc finger protein. SCG10, a pan-neuronal membrane protein, is first detected one stage later, followed by the catecholaminergic neurotransmitter enzyme tyrosine hydroxylase (TH). We have used these markers to ask two questions: (1) is their expression dependent upon inductive signals derived from the notochord or floor plate?; (2) does their sequential expression reflect a single linear pathway or multiple parallel pathways? Notochord ablation experiments indicate that the floor plate is essential for induction of GATA-2, Phox2 and TH, but not for that of Cash-1 and SCG10. Taken together these data suggest that the development of sympathetic neurons involves multiple transcriptional regulatory cascades: one, dependent upon notochord or floor plate-derived signals and involving Phox2 and GATA-2, is assigned to the expression of the neurotransmitter phenotype; the other, independent of such signals and involving Cash-1, is assigned to the expression of pan-neuronal properties. The parallel specification of different components of the terminal neuronal phenotype is likely to be a general feature of neuronal development.

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Anderson, David J.0000-0001-6175-3872
Additional Information:© 1995 by Company of Biologists. (Accepted 10 November 1994) We are grateful to Dr Tom Reh for providing chick Cash-1 cDNA clones in advance of publication, and to Dr Christo Goridis for communicating results on Phox2 in advance of publication and for his useful comments on the manuscript. We wish to thank Dr Tom Jessell and Dr Hiroshi Hatanaka for gifts of the FP1 and PCTH-7 antibodies respectively, and to Dr Peter Jeffrey for his gift of the chicken SCG10 cDNA. A.K.G. is grateful to Cheryll Tickle for introducing him to chick embryology, and to Claudio Stern for demonstrating notochord removal. We would like to thank Kai Zinn for the use of his microscopy facilities, Steve Padilla for technical assistance and Liching Lo for advice on the in situ hybridization procedure. This work was supported by a Long Term Fellowship from the Human Frontiers Science Program Organization (A.K.G.), NIH grant GM28896 (J.D.E.), NIH training grant GM08061 (K.M.G.), NIH grant NS23476 (D.J.A.) and by grants from the Association pour la Recherche Contre le Cancer and the Association Française contre les Myopathies (J.-F.B.). D.J.A. is an Associate Investigator of the Howard Hughes Medical Institute.
Funding AgencyGrant Number
Human Frontier Science ProgramUNSPECIFIED
NIHGM 28896
NIHGM 08061
Association pour la Recherche Contre le CancerUNSPECIFIED
Association Française contre les MyopathiesUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Subject Keywords:transcription factor gene, gene expression, phenotypic marker, neuron, sympathetic neuron, GATA-2, Phox2, TH, Cash-1, SCG10
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Record Number:CaltechAUTHORS:GROdev95
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ID Code:12786
Deposited By: Archive Administrator
Deposited On:23 Dec 2008 21:54
Last Modified:30 Mar 2020 22:14

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