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Molecular organization of Gram-negative peptidoglycan

Gan, Lu and Chen, Songye and Jensen, Grant J. (2008) Molecular organization of Gram-negative peptidoglycan. Proceedings of the National Academy of Sciences of the United States of America, 105 (48). pp. 18953-18957. ISSN 0027-8424. PMCID PMC2596242. https://resolver.caltech.edu/CaltechAUTHORS:GANpnas08

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Abstract

The stress-bearing component of the bacterial cell wall -— a multi-gigadalton bag-like molecule called the sacculus -— is synthesized from peptidoglycan. Whereas the chemical composition and the 3-dimensional structure of the peptidoglycan subunit (in at least one conformation) are known, the architecture of the assembled sacculus is not. Four decades' worth of biochemical and electron microscopy experiments have resulted in two leading 3-D peptidoglycan models: “Layered” and “Scaffold”, in which the glycan strands are parallel and perpendicular to the cell surface, respectively. Here we resolved the basic architecture of purified, frozen-hydrated sacculi through electron cryotomography. In the Gram-negative sacculus, a single layer of glycans lie parallel to the cell surface, roughly perpendicular to the long axis of the cell, encircling the cell in a disorganized hoop-like fashion.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.0808035105DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2596242/PubMed CentralArticle
http://www.pnas.org/cgi/content/full/0808035105/DCSupplemental.PublisherSupporting Information
ORCID:
AuthorORCID
Gan, Lu0000-0002-8685-4896
Chen, Songye0000-0001-5407-5049
Jensen, Grant J.0000-0003-1556-4864
Additional Information:© 2008 by the National Academy of Sciences. Edited by M. J. Osborn, University of Connecticut Health Center, Farmington, CT, and approved October 9, 2008 (received for review August 14, 2008). Published online before print November 24, 2008, doi:10.1073/pnas.0808035105 We thank Drs. W. F. Tivol for help with EM; H. Tsuruta and M. Hammel for help at beamlines SSRL 4-2 and ALS 12.3.1, respectively; A. Briegel, D.P. Dias, and Z. Li for sharing data; J. Skerker, K.C. Huang, N. Wingreen, and P. Leong for discussions; and Mr. Everett Kane (SuperSoft Design, New York, NY) for Fig. 1C. This work was supported in part by National Institutes of Health Grants R01 AI067548 and P50 GM082545 to G.J.J., a Searle Scholar Award to G.J.J., the Beckman Institute at Caltech, and gifts to Caltech from the Gordon and Betty Moore Foundation and the Agouron Institute. L.G. is a Damon Runyon Fellow supported by a fellowship from the Damon Runyon Cancer Research Foundation (DRG-1940-07). Author contributions: L.G. and G.J.J. designed research; L.G. and S.C. performed research; L.G., S.C., and G.J.J. analyzed data; and L.G. and G.J.J. wrote the paper. The authors declare no conflict of interest. This article is a PNAS Direct Submission. This article contains supporting information online at www.pnas.org/cgi/content/full/0808035105/DCSupplemental.
Funders:
Funding AgencyGrant Number
NIHR01 AI067548
NIHP50 GM082545
Searle Scholars ProgramUNSPECIFIED
Caltech Beckman InstituteUNSPECIFIED
Gordon and Betty Moore FoundationUNSPECIFIED
Agouron InstituteUNSPECIFIED
Damon Runyon Cancer Research FoundationDRG-1940-07
Subject Keywords:cell wall; Cryo-EM; sacculus; tomography; cell shape
Issue or Number:48
PubMed Central ID:PMC2596242
Record Number:CaltechAUTHORS:GANpnas08
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:GANpnas08
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:12835
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:06 Jan 2009 06:35
Last Modified:27 Apr 2020 22:09

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