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Pattern formation during de novo assembly of the Arabidopsis shoot meristem

Gordon, Sean P. and Heisler, Marcus G. and Reddy, G. Venugopala and Ohno, Carolyn and Das, Pradeep and Meyerowitz, Elliot M. (2007) Pattern formation during de novo assembly of the Arabidopsis shoot meristem. Development, 134 (19). pp. 3539-3548. ISSN 0950-1991. https://resolver.caltech.edu/CaltechAUTHORS:GORdev07

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Abstract

Most multicellular organisms have a capacity to regenerate tissue after wounding. Few, however, have the ability to regenerate an entire new body from adult tissue. Induction of new shoot meristems from cultured root explants is a widely used, but poorly understood, process in which apical plant tissues are regenerated from adult somatic tissue through the de novo formation of shoot meristems. We characterize early patterning during de novo development of the Arabidopsis shoot meristem using fluorescent reporters of known gene and protein activities required for shoot meristem development and maintenance. We find that a small number of progenitor cells initiate development of new shoot meristems through stereotypical stages of reporter expression and activity of CUP-SHAPED COTYLEDON 2 (CUC2), WUSCHEL (WUS), PIN-FORMED 1 (PIN1), SHOOT-MERISTEMLESS (STM), FILAMENTOUS FLOWER (FIL, also known as AFO), REVOLUTA (REV), ARABIDOPSIS THALIANA MERISTEM L1 LAYER (ATML1) and CLAVATA 3 (CLV3). Furthermore, we demonstrate a functional requirement for WUS activity during de novo shoot meristem initiation. We propose that de novo shoot meristem induction is an easily accessible system for the study of patterning and self-organization in the well-studied model organism Arabidopsis.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1242/10.1242/dev.010298DOIArticle
http://dev.biologists.org/cgi/content/abstract/134/19/3539PublisherArticle
ORCID:
AuthorORCID
Heisler, Marcus G.0000-0001-5644-8398
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© The Company of Biologists Ltd 2007. Accepted 21 July 2007. First published online September 7, 2007 doi: 10.1242/10.1242/dev.010298 We thank Joe Keiber for the ARR5::GFP seeds. We thank Kaoru Sugimoto for assistance with the supplemental RT-PCR data, Annick Dubois and Arnavaz Garda for technical advice, and Elizabeth Haswell for critical comments on the manuscript. This work was funded by National Science Foundation grant IOS-0211670 to E.M.M. Supplementary material for this article is available at http://dev.biologists.org/cgi/content/full/134/19/3539/DC1
Funders:
Funding AgencyGrant Number
NSFIOS-0211670
Subject Keywords:Auxin, Callus, Cytokinin, Regeneration, Self-organization, Shoot meristem, Arabidopsis thaliana
Issue or Number:19
Record Number:CaltechAUTHORS:GORdev07
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:GORdev07
Official Citation:Pattern formation during de novo assembly of the Arabidopsis shoot meristem Sean P. Gordon, Marcus G. Heisler, G. Venugopala Reddy, Carolyn Ohno, Pradeep Das, Elliot M. Meyerowitz Development 2007 134: 3539-3548; doi: 10.1242/dev.010298
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:12960
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:11 Jan 2009 05:40
Last Modified:03 Oct 2019 00:33

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