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Comprehensive characterization of molecular interactions based on nanomechanics

Ghatkesar, Murali Krishna and Lang, Hans-Peter and Gerber, Christoph and Hegner, Martin and Braun, Thomas (2008) Comprehensive characterization of molecular interactions based on nanomechanics. PLoS ONE, 3 (11). Art. No. e3610. ISSN 1932-6203. PMCID PMC2572191. https://resolver.caltech.edu/CaltechAUTHORS:GHAplosone08

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Abstract

Molecular interaction is a key concept in our understanding of the biological mechanisms of life. Two physical properties change when one molecular partner binds to another. Firstly, the masses combine and secondly, the structure of at least one binding partner is altered, mechanically transducing the binding into subsequent biological reactions. Here we present a nanomechanical micro-array technique for bio-medical research, which not only monitors the binding of effector molecules to their target but also the subsequent effect on a biological system in vitro. This label-free and real-time method directly and simultaneously tracks mass and nanomechanical changes at the sensor interface using micro-cantilever technology. To prove the concept we measured lipid vesicle (approximately 748*10(6) Da) adsorption on the sensor interface followed by subsequent binding of the bee venom peptide melittin (2840 Da) to the vesicles. The results show the high dynamic range of the instrument and that measuring the mass and structural changes simultaneously allow a comprehensive discussion of molecular interactions.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1371/journal.pone.0003610DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2572191/PubMed CentralArticle
Additional Information:© 2008 Ghatkesar et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received July 21, 2008; Accepted: October 8, 2008; Published: November 3, 2008. We thank Jean-Pierre Ramseyer and Wilfried Grange for their general help and discussions. Ekatherina Rakhmatullina for her expert help in dynamic light scattering, Christian Dittrich and Wolfgang Meier for providing their extrusion set-up (all University Basel, Switzerland). We thank Ronan Daly, Trinity College Dublin, Ireland for carefully reading the manuscript. Financial support is acknowledged from SNF (NCCR Nanoscale Science, Switzerland), the Cleven-Becker Stiftung, Endress Foundation (Switzerland), and the ELTEM Regio and SFI (Ireland). Author MKG would like to thank Swiss National Science Foundation (SNSF) and Novartis Foundation, Switzerland for the research fellowship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Author Contributions: Conceived and designed the experiments: TB. Performed the experiments: MKG. Analyzed the data: MKG. Contributed reagents/materials/analysis tools: HPL CG MH TB. Wrote the paper: MKG HPL TB. Software for data analysis: TB. Competing interests: The authors have declared that no competing interests exist.
Funders:
Funding AgencyGrant Number
Swiss National Science Foundation (SNSF)UNSPECIFIED
Cleven-Becker StiftungUNSPECIFIED
Endress FoundationUNSPECIFIED
Science Foundation, IrelandUNSPECIFIED
Novartis FoundationUNSPECIFIED
Issue or Number:11
PubMed Central ID:PMC2572191
Record Number:CaltechAUTHORS:GHAplosone08
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:GHAplosone08
Usage Policy:This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ID Code:13282
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:09 Feb 2009 22:15
Last Modified:12 Feb 2020 21:32

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