Tivol, William F. and Briegel, Ariane and Jensen, Grant J. (2008) An improved cryogen for plunge freezing. Microscopy and Microanalysis, 14 (5). pp. 375-379. ISSN 1431-9276. PMCID PMC3058946. doi:10.1017/S1431927608080781. https://resolver.caltech.edu/CaltechAUTHORS:TIVmm08
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Abstract
The use of an alkane mixture that remains liquid at 77 K to freeze specimens has advantages over the use of a pure alkane that is solid at 77 K. It was found that a mixture of methane and ethane did not give a cooling rate adequate to produce vitreous ice, but a mixture of propane and ethane did result in vitreous ice. Furthermore, the latter mixture produced less damage to specimens mounted on a very thin, fragile holey carbon substrate.
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| Additional Information: | © Microscopy Society of America 2008. Received December 20, 2007; accepted June 4, 2008. This work was supported in part by NIH grants R01 AI067548 and P50 GM082545 to G.J.J., a Searle Scholar Award to G.J.J., the Beckman Institute at Caltech, and gifts to Caltech from the Gordon and Betty Moore Foundation and Agouron Institute. | ||||||||||||||
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| Subject Keywords: | cryogen; plunge freezing; electron cryomicroscopy; ice embedding; transmission electron microscopy; specimen preparation | ||||||||||||||
| Issue or Number: | 5 | ||||||||||||||
| PubMed Central ID: | PMC3058946 | ||||||||||||||
| DOI: | 10.1017/S1431927608080781 | ||||||||||||||
| Record Number: | CaltechAUTHORS:TIVmm08 | ||||||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:TIVmm08 | ||||||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||||
| ID Code: | 13355 | ||||||||||||||
| Collection: | CaltechAUTHORS | ||||||||||||||
| Deposited By: | Tony Diaz | ||||||||||||||
| Deposited On: | 23 Apr 2009 18:32 | ||||||||||||||
| Last Modified: | 08 Nov 2021 22:37 |
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