A Caltech Library Service

An improved cryogen for plunge freezing

Tivol, William F. and Briegel, Ariane and Jensen, Grant J. (2008) An improved cryogen for plunge freezing. Microscopy and Microanalysis, 14 (5). pp. 375-379. ISSN 1431-9276. PMCID PMC3058946. doi:10.1017/S1431927608080781.

PDF - Published Version
See Usage Policy.


Use this Persistent URL to link to this item:


The use of an alkane mixture that remains liquid at 77 K to freeze specimens has advantages over the use of a pure alkane that is solid at 77 K. It was found that a mixture of methane and ethane did not give a cooling rate adequate to produce vitreous ice, but a mixture of propane and ethane did result in vitreous ice. Furthermore, the latter mixture produced less damage to specimens mounted on a very thin, fragile holey carbon substrate.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Briegel, Ariane0000-0003-3733-3725
Jensen, Grant J.0000-0003-1556-4864
Additional Information:© Microscopy Society of America 2008. Received December 20, 2007; accepted June 4, 2008. This work was supported in part by NIH grants R01 AI067548 and P50 GM082545 to G.J.J., a Searle Scholar Award to G.J.J., the Beckman Institute at Caltech, and gifts to Caltech from the Gordon and Betty Moore Foundation and Agouron Institute.
Funding AgencyGrant Number
NIHR01 AI067548
NIHP50 GM082545
Searle Scholar AwardUNSPECIFIED
Caltech Beckman InstituteUNSPECIFIED
Gordon and Betty Moore FoundationUNSPECIFIED
Agouron InstituteUNSPECIFIED
Subject Keywords:cryogen; plunge freezing; electron cryomicroscopy; ice embedding; transmission electron microscopy; specimen preparation
Issue or Number:5
PubMed Central ID:PMC3058946
Record Number:CaltechAUTHORS:TIVmm08
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:13355
Deposited By: Tony Diaz
Deposited On:23 Apr 2009 18:32
Last Modified:08 Nov 2021 22:37

Repository Staff Only: item control page