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An improved cryogen for plunge freezing

Tivol, William F. and Briegel, Ariane and Jensen, Grant J. (2008) An improved cryogen for plunge freezing. Microscopy and Microanalysis, 14 (5). pp. 375-379. ISSN 1431-9276. PMCID PMC3058946. https://resolver.caltech.edu/CaltechAUTHORS:TIVmm08

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Abstract

The use of an alkane mixture that remains liquid at 77 K to freeze specimens has advantages over the use of a pure alkane that is solid at 77 K. It was found that a mixture of methane and ethane did not give a cooling rate adequate to produce vitreous ice, but a mixture of propane and ethane did result in vitreous ice. Furthermore, the latter mixture produced less damage to specimens mounted on a very thin, fragile holey carbon substrate.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1017/S1431927608080781DOIArticle
http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=2203280PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3058946/PubMed CentralArticle
ORCID:
AuthorORCID
Briegel, Ariane0000-0003-3733-3725
Jensen, Grant J.0000-0003-1556-4864
Additional Information:© Microscopy Society of America 2008. Received December 20, 2007; accepted June 4, 2008. This work was supported in part by NIH grants R01 AI067548 and P50 GM082545 to G.J.J., a Searle Scholar Award to G.J.J., the Beckman Institute at Caltech, and gifts to Caltech from the Gordon and Betty Moore Foundation and Agouron Institute.
Funders:
Funding AgencyGrant Number
NIHR01 AI067548
NIHP50 GM082545
Searle Scholar AwardUNSPECIFIED
Caltech Beckman InstituteUNSPECIFIED
Gordon and Betty Moore FoundationUNSPECIFIED
Agouron InstituteUNSPECIFIED
Subject Keywords:cryogen; plunge freezing; electron cryomicroscopy; ice embedding; transmission electron microscopy; specimen preparation
Issue or Number:5
PubMed Central ID:PMC3058946
Record Number:CaltechAUTHORS:TIVmm08
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:TIVmm08
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:13355
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Apr 2009 18:32
Last Modified:03 Oct 2019 00:36

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