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Dynamic allosteric control of noncovalent DNA catalysis reactions

Zhang, David Yu and Winfree, Erik (2008) Dynamic allosteric control of noncovalent DNA catalysis reactions. Journal of the American Chemical Society, 130 (42). pp. 13921-13926. ISSN 0002-7863. doi:10.1021/ja803318t.

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Allosteric modulation of catalysis kinetics is prevalent in proteins and has been rationally designed for ribozymes. Here, we present an allosteric DNA molecule that, in its active configuration, catalyzes a noncovalent DNA reaction. The catalytic activity is designed to be modulated by the relative concentrations of two DNA regulator molecules, one an inhibitor and the other an activator. Dynamic control of the catalysis rate is experimentally demonstrated via three cycles of up and down regulation by a factor of over 10. Unlike previous works, both the allosteric receptor and catalytic core are designed, rather than evolved. This allows flexibility in the sequence design and modularity in synthetic network construction.

Item Type:Article
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URLURL TypeDescription
Winfree, Erik0000-0002-5899-7523
Additional Information:© 2008 American Chemical Society. Received May 5, 2008. The authors were supported by NSF grants 062254 and 0728703 to E.W. D.Y.Z. is supported by the Fannie and John Hertz Foundation.
Funding AgencyGrant Number
Fannie and John Hertz FoundationUNSPECIFIED
Subject Keywords:gene-expression; rational design; RNA; ribozymes; molecules; switches; kinetics
Issue or Number:42
Record Number:CaltechAUTHORS:ZHAjacs08
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:13360
Deposited By: Tony Diaz
Deposited On:23 Apr 2009 20:28
Last Modified:08 Nov 2021 22:37

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