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Internally calibrated quantification of protein analytes in human serum by fluorescence immunoassays in disposable elastomeric microfluidic devices

Kartalov, Emil P. and Lin, David H. and Lee, David T. and Anderson, William F. and Taylor, Clive R. and Scherer, Axel (2008) Internally calibrated quantification of protein analytes in human serum by fluorescence immunoassays in disposable elastomeric microfluidic devices. Electrophoresis, 29 (24). pp. 5010-5016. ISSN 1522-2683. http://resolver.caltech.edu/CaltechAUTHORS:KARe08

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Abstract

Herein we report on reliable reproducible quantification of protein analytes in human serum by fluorescence sandwich immunoassays in disposable PDMS microfluidic chips. The system requires 1000 times less sample than typical clinical blood tests and is specifically shown to measure ferritin down to 250 pM in human serum. The in-built calibration method of spiking the serum with known concentrations of commercially available antigen avoids common sources of error and improves the reliability of the test results. The reported microfluidic system is an important new tool for fundamental scientific research, offering sensitive immunoassay measurements in small but complex biosamples. The system is also a further step towards comprehensive affordable "point-of-care" biomedical diagnostics.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/elps.200800297DOIUNSPECIFIED
http://www3.interscience.wiley.com/journal/121616998/abstractPublisherUNSPECIFIED
Additional Information:© 2008 WILEY-VCH Verlag GmbH & Co. Received May 13, 2008, Revised June 19, 2008, Accepted June 23, 2008. The authors thank Christina Morales and Anna Chetverikova of the Caltech Microfluidic Foundry for their help with device fabrication. The authors also thank Avril Cunningham of the USC Leavy Library for her help with the reference material. Financial support for this work was provided by NIH Grants 1K99EB007151 and 1RO1HG002644 and the Caltech SURF Program. The authors have declared no conflict of interest.
Group:Kavli Nanoscience Institute
Funders:
Funding AgencyGrant Number
National Institutes of Health1K99EB007151
National Institutes of Health1RO1HG002644
Caltech SURFUNSPECIFIED
Subject Keywords:Diagnostics; Fluorescence; Immunoassay; Microfluidic; Serum
Record Number:CaltechAUTHORS:KARe08
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:KARe08
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:13418
Collection:CaltechAUTHORS
Deposited By: Kristin Buxton
Deposited On:17 Apr 2009 16:52
Last Modified:26 Dec 2012 10:49

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