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Phase-contrast OCT imaging of transverse flows in the mouse retina and choroid

Fingler, J. and Readhead, C. and Schwartz, D. M. and Fraser, S. E. (2008) Phase-contrast OCT imaging of transverse flows in the mouse retina and choroid. Investigative Ophthalmology and Visual Science, 49 (11). pp. 5055-5059. ISSN 0146-0404.

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PURPOSE. To test the hypothesis that a novel phase-contrast optical coherence tomography (OCT) system can image retinal and choroidal vessels in the living mouse. METHODS. A high-speed spectral domain optical coherence tomography (SDOCT) system, which measures the reflections for the entire depth of the retina at once with each axial scan (A-scan), was developed for mouse retinal imaging. Acquiring multiple A-scans over a transverse line across the mouse retina offers a two-dimensional cross-sectional image (B-scan); several neighboring B-scans can be assembled into a three-dimensional OCT image. To visualize mobility and transverse flow in retinal vessels, the statistical variance of phase for each location was calculated from multiple B-scans acquired successively for the same retinal cross-section. Such measures of phase variance offer a direct measure of motions over a large dynamic range of flow velocities. RESULTS. Three-dimensional phase-contrast images of the live mouse retina were created using multiple two-dimensional cross-sectional image slices through the retina. For the data presented here, each cross-sectional phase-contrast slice resulted from five images of 100 or 200 transverse pixels, acquired over 25 ms or 50 ms, respectively. The approach offered clear identification of motion regions at different depths, including flow in the retinal microvasculature and in the choroidal vessels. Phase contrast OCT enables three-dimensional visualization of retinal and choroidal vasculature in vivo.

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Fraser, S. E.0000-0002-5377-0223
Additional Information:© 2008 Association for Research in Vision and Ophthalmology. Submitted for publication December 18, 2007; revised February 17 and June 6, 2008; accepted September 15, 2008. The authors thank Carl Zeiss Meditec for generously providing technical and material assistance in the development of the imaging system.
Funding AgencyGrant Number
Lincy FoundationUNSPECIFIED
National Eye InstituteEY02162
Hellman FoundationUNSPECIFIED
Research to Prevent BlindnessUNSPECIFIED
Issue or Number:11
Record Number:CaltechAUTHORS:FINiovs08
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:13554
Deposited By: Ruth Sustaita
Deposited On:17 Jun 2009 21:31
Last Modified:03 Oct 2019 00:40

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