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Epibiotic bacteria associated with the recently discovered Yeti crab, Kiwa hirsuta

Goffredi, Shana K. and Jones, William J. and Erhlich, Hermann and Springer, Armin and Vrijenhoek, Robert C. (2008) Epibiotic bacteria associated with the recently discovered Yeti crab, Kiwa hirsuta. Environmental Microbiology, 10 (10). pp. 2623-2634. ISSN 1462-2912. https://resolver.caltech.edu/CaltechAUTHORS:GOFem08

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Abstract

The Yeti crab, Kiwa hirsuta Macpherson et al., is the single known species in a recently discovered crab family Kiwaidae (Decapoda: Galatheoidea) from deepsea hydrothermal vents. Its chelipeds, walking legs and the ventral surface of its cephalothorax are covered with dense setae that, in turn, are covered with clusters of filamentous bacteria, making the crab appear extraordinarily ‘hairy’. Electron microscopy revealed dense bacterial clusters attached to the chitinous outer layer of the setae. Molecular phylogenetic analyses revealed the setae-associated bacteria to be dominated by e-Proteobacteria (~56% of the recovered ribotypes), g-Proteobacteria (~25%) and Bacteroidetes (~10%). Fluorescence in situ microscopy confirmed the attachment of filamentous e-Proteobacteria on setae, but no specialized morphological structures appeared to exist for bacterial attachment. Key enzymes involved in the reductive tricarboxylic acid cycle (ATP-dependent citrate lyase) and sulfite oxidation or dissimilatory sulfate reduction (bidirectional APS reductase) were detected. Consequently, the potential for carbon fixation and cycling of reduced and oxidized sulfur appear to exist in the dense microflora that grows on the crab’s setae.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1111/j.1462-2920.2008.01684.xDOIArticle
ORCID:
AuthorORCID
Goffredi, Shana K.0000-0002-9110-9591
Additional Information:© 2008 The Authors. Received 24 February, 2008; accepted 10 May, 2008.We thank Michel Segonzac for generously providing setae from the holotype specimen of K. hirsuta. This work would not have been possible without the expertise of DSRV Alvin pilots, particularly Gavin Eppard, who sampled the holotype specimen. The RV Atlantis crew and a number of scientific participants, particularly W.J. Jones and S. Johnson, provided critical shipboard support for this project. K. Buck generated the image in Fig. 1B. A. Pernthaler provided advice regarding FISH analyses and contributed to image in Fig. 2H. J. Jackson and V. Orphan provided laboratory space and the use of microscopy and DNA sequencing facilities at the California Institute of Technology. Research was supported by grants from the US National Science Foundation (MCB-0454860 to S.K.G. and OCE-0241613 to R.C.V.) and from the David and Lucile Packard Foundation to MBARI.
Funders:
Funding AgencyGrant Number
NSFMCB-0454860
NSFOCE-0241613
David and Lucile Packard FoundationUNSPECIFIED
Subject Keywords:SEA HYDROTHERMAL VENTS; 16S RIBOSOMAL-RNA; SULFATE-REDUCING PROKARYOTES; HIGHLY SPECIFIC ASSOCIATION; IN-SITU HYBRIDIZATION; MID-ATLANTIC RIDGE; REDUCTASE GENES; MARINE-BACTERIA; PHYLOGENETIC ANALYSIS; ALVINELLA-POMPEJANA.
Issue or Number:10
Record Number:CaltechAUTHORS:GOFem08
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:GOFem08
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:13562
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:16 Jun 2009 18:15
Last Modified:03 Oct 2019 23:39

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