CaltechAUTHORS
  A Caltech Library Service

Mechanism of cellular uptake of a ruthenium polypyridyl complex

Puckett, Cindy A. and Barton, Jacqueline K. (2008) Mechanism of cellular uptake of a ruthenium polypyridyl complex. Biochemistry, 47 (45). pp. 11711-11716. ISSN 0006-2960. PMCID PMC2747514. http://resolver.caltech.edu/CaltechAUTHORS:PUCb08

[img] PDF - Accepted Version
See Usage Policy.

2723Kb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:PUCb08

Abstract

Transition metal complexes provide a promising avenue for the design of therapeutic and diagnostic agents, but the limited understanding of their cellular uptake is a roadblock to their effective application. Here, we examine the mechanism of cellular entry of a luminescent ruthenium(II) polypyridyl complex, Ru(DIP)2dppz2+ (where DIP = 4,7-diphenyl-1,10-phenanthroline and dppz = dipyridophenazine), into HeLa cells, with the extent of uptake measured by flow cytometry. No diminution of cellular uptake is observed under metabolic inhibition with deoxyglucose and oligomycin, indicating an energy-independent mode of entry. The presence of organic cation transporter inhibitors also does not significantly alter uptake. However, the cellular internalization of Ru(DIP)2dppz2+ is sensitive to the membrane potential. Uptake decreases when cells are depolarized with high potassium buffer and increases when cells are hyperpolarized with valinomycin. These results support passive diffusion of Ru(DIP)2dppz2+ into the cell.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/bi800856tDOIArticle
http://pubs.acs.org/doi/abs/10.1021/bi800856tPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2747514/PubMed CentralArticle
ORCID:
AuthorORCID
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2008 American Chemical Society. Received May 9, 2008; Revised manuscript received September 4, 2008. We are grateful to the Caltech Flow Cytometry Facility for expert assistance and facilities. Financial support for this work from the National Institutes of Health (Grant GM33309 to J.K.B.) is acknowledged.
Funders:
Funding AgencyGrant Number
NIHGM33309
Subject Keywords:organic cation transporters; mediated drug-delivery; light switch; cells; receptor; DNA; intercalators; mitochondrial; sequence
PubMed Central ID:PMC2747514
Record Number:CaltechAUTHORS:PUCb08
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:PUCb08
Official Citation:Mechanism of Cellular Uptake of a Ruthenium Polypyridyl Complex† Cindy A. Puckett and Jacqueline K. Barton Biochemistry 2008 47 (45), 11711-11716 DOI: 10.1021/bi800856t
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:13578
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:11 May 2009 17:19
Last Modified:26 Jan 2016 01:42

Repository Staff Only: item control page