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An all-optical approach for probing microscopic flows in living embryos

Supatto, Willy and Fraser, Scott E. and Vermot, Julien (2008) An all-optical approach for probing microscopic flows in living embryos. Biophysical Journal, 95 (4). L29-L31. ISSN 0006-3495. https://resolver.caltech.edu/CaltechAUTHORS:SUPbpj08

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Abstract

Living systems rely on fluid dynamics from embryonic development to adulthood. To visualize biological fluid flow, devising the proper labeling method compatible with both normal biology and in vivo imaging remains a major experimental challenge. Here, we describe a simple strategy for probing microscopic fluid flows in vivo that meets this challenge. An all-optical procedure combining femtosecond laser ablation, fast confocal microscopy and 3D-particle tracking was devised to label, image and quantify the flow. This approach is illustrated by studying the flow generated within a micrometer scale ciliated vesicle located deep inside the zebrafish embryo and involved in breaking left-right embryonic symmetry. By mapping the velocity field within the vesicle and surrounding a single beating cilium, we show this method can address the dynamics of cilia-driven flows at multiple length scales, and can validate the flow features as predicted from previous simulations. This approach provides new experimental access to questions of microscopic fluid dynamics in vivo.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1529/biophysj.108.137786DOIArticle
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5377-0223
Additional Information:© 2008 Biophysical Society. Received for publication 16 May 2008 and in final form 6 June 2008. We are grateful to D. Wu and T. Truong for critical comments, the Caltech Biological Imaging Center, and P. Björkman for sharing equipments. J.V. was supported by the Human Frontier Science Program Fellowship. This work was supported by the Biological Imaging Center of the Caltech Beckman Institute and a Centers of Excellence in Genomic Sciences grant from the National Institutes of Health (P50 HG004071).
Funders:
Funding AgencyGrant Number
Human Frontier Science ProgramUNSPECIFIED
Caltech Beckman InstituteUNSPECIFIED
NIHP50 HG004071
Subject Keywords:LEFT-RIGHT ASYMMETRY; FEMTOSECOND LASER; NODAL FLOW; KUPFFERS VESICLE; MECHANICS; CILIA; BRAIN; HEART
Issue or Number:4
Record Number:CaltechAUTHORS:SUPbpj08
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:SUPbpj08
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:13817
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:01 Apr 2009 20:20
Last Modified:03 Oct 2019 00:44

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