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A membrane associated mCherry fluorescent reporter line for studying vascular remodeling and cardiac function during murine embryonic development

Larina, Irina V. and Shen, Wei and Kelly, Olivia G. and Hadjantonakis, Anna-Katerina and Baron, Margaret H. and Dickinson, Mary E. (2009) A membrane associated mCherry fluorescent reporter line for studying vascular remodeling and cardiac function during murine embryonic development. Anatomical Record, 292 (3). pp. 333-341. ISSN 1932-8486. https://resolver.caltech.edu/CaltechAUTHORS:20090410-094507129

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Abstract

The development of the cardiovascular system is a highly dynamic process dependent on multiple signaling pathways regulating proliferation, differentiation, migration, cell-cell and cell-matrix interactions. To characterize cell and tissue dynamics during the formation of the cardiovascular system in mice, we generated a novel transgenic mouse line, Tg(Flk1::myr-mCherry), in which endothelial cell membranes are brightly labeled with mCherry, a red fluorescent protein. Tg(Flk1::myr-mCherry) mice are viable, fertile, and do not exhibit any developmental abnormalities. High levels of mCherry are expressed in the embryonic endothelium and endocardium, and expression is also observed in capillaries in adult animals. Targeting of the fluorescent protein to the cell membrane allows for subcellular imaging and cell tracking. By acquiring confocal time lapses of live embryos cultured on the microscope stage, we demonstrate that the newly generated transgenic model beautifully highlights the sprouting behaviors of endothelial cells during vascular plexus formation. We have also used embryos from this line to imaging the endocardium in the beating embryonic mouse heart, showing that Tg(Flk1::myr-mCherry) mice are suitable for the characterization of cardio dynamics. Furthermore, when combined with the previously described Tg(Flk1::H2B-EYFP) line, cell number in addition to cell architecture is revealed, making it possible to determine how individual endothelial cells contribute to the structure of the vessel.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/ar.20821DOIUNSPECIFIED
http://www3.interscience.wiley.com/journal/122220832/abstractPublisherUNSPECIFIED
Additional Information:© 2009 Wiley-Liss, Inc. Received: 30 January 2008; Accepted: 6 June 2008. Grant sponsor: American Heart Association; Grant number: 0625187Y; Grant sponsor: National Institutes of Health; Grant numbers: RO1 HL077187, RO1 EB02209.
Funders:
Funding AgencyGrant Number
American Heart Association0625187Y
NIHRO1 HL077187
NIHRO1 EB02209
Subject Keywords:live imaging; mCherry; Flk1; heart dynamics; vasculogenesis; angiogenesis; embryonic development; cell tracking; yolk sac
Issue or Number:3
Record Number:CaltechAUTHORS:20090410-094507129
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20090410-094507129
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:13922
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:03 Aug 2009 21:03
Last Modified:03 Oct 2019 00:45

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