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Floral stem cell termination involves the direct regulation of AGAMOUS by PERIANTHIA

Das, Pradeep and Ito, Toshiro and Wellmer, Frank and Vernoux, Teva and Dedieu, Annick and Traas, Jan and Meyerowitz, Elliot M. (2009) Floral stem cell termination involves the direct regulation of AGAMOUS by PERIANTHIA. Development, 136 (10). pp. 1605-1611. ISSN 0950-1991. doi:10.1242/10.1242/dev.035436. https://resolver.caltech.edu/CaltechAUTHORS:20090609-104852613

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[img] PDF (Fig. S1. Interactions between pan and the weak lfy-5 allele.) - Supplemental Material
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[img] PDF (Fig. S2. In situ localizations of WUS mRNA in pan flowers.) - Supplemental Material
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[img] PDF (Fig. S3. In situ localizations of WUS mRNA in lfy flowers.) - Supplemental Material
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[img] PDF (Fig. S4. In situ localizations of WUS mRNA in lfy pan double mutants.) - Supplemental Material
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PDF (Fig. S5. pan mutants reveal determinacy defects under sensitized conditions.) - Supplemental Material
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[img] PDF (Fig. S6. Interactions between pan mutants and mutants with fourth-whorl phenotypes.) - Supplemental Material
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[img] PDF (Fig. S7. The AP1(1.7-kb) promoter fragment drives expression throughout the flower.) - Supplemental Material
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[img] PDF (Fig. S8. An inducible pPAN::alcR−alcA::PAN-RD produces causes floral indeterminacy.) - Supplemental Material
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[img] PDF (Fig. S9. Results of particle bombardment experiments.) - Supplemental Material
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Abstract

In Arabidopsis, the population of stem cells present in young flower buds is lost after the production of a fixed number of floral organs. The precisely timed repression of the stem cell identity gene WUSCHEL (WUS) by the floral homeotic protein AGAMOUS (AG) is a key part of this process. In this study, we report on the identification of a novel input into the process of floral stem cell regulation. We use genetics and chromatin immunoprecipitation assays to demonstrate that the bZIP transcription factor PERIANTHIA (PAN) plays a role in regulating stem cell fate by directly controlling AG expression and suggest that this activity is spatially restricted to the centermost region of the AG expression domain. These results suggest that the termination of floral stem cell fate is a multiply redundant process involving loci with unrelated floral patterning functions.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1242/10.1242/dev.035436DOIArticle
http://dev.biologists.org/cgi/content/abstract/136/10/1605PublisherArticle
ORCID:
AuthorORCID
Ito, Toshiro0000-0002-8206-2787
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2009 The Company of Biologists Ltd. Accepted 16 March 2009. We thank J. Lohmann for sharing unpublished results; Arnavaz Garda, Alexis Lacroix, Claudia Bardoux and Hervé Leyral for technical assistance; Ioan Negrutiu, Christophe Trehin, Nathanaël Prunet and Olivier Hamant for useful discussions and comments on the manuscript; Ioan Negrutiu for sharing unpublished results and seeds; and John Bowman and Marty Yanofsky for communicating unpublished results. This work was supported by a Caltech Division of Biology postdoctoral fellowship (to P.D.), a Marie Curie Incoming International Fellowship IIF-022002 (to P.D.), the EU Marie Curie SY-STEM Network (to J.T.) and a NSF Grant IOS-0544915 (to E.M.M.).
Funders:
Funding AgencyGrant Number
Caltech Division of BiologyUNSPECIFIED
Marie Curie FellowshipIIF-022002
NSFIOS-0544915
Subject Keywords:AGAMOUS; flower development; stem cells; arabidopsis
Issue or Number:10
DOI:10.1242/10.1242/dev.035436
Record Number:CaltechAUTHORS:20090609-104852613
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20090609-104852613
Official Citation:Floral stem cell termination involves the direct regulation of AGAMOUS by PERIANTHIA Pradeep Das, Toshiro Ito, Frank Wellmer, Teva Vernoux, Annick Dedieu, Jan Traas, Elliot M. Meyerowitz Development 2009 136: 1605-1611; doi: 10.1242/dev.035436
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:14377
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 Jul 2009 21:07
Last Modified:08 Nov 2021 23:11

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