A Caltech Library Service

A proposal for a coordinated effort for the determination of brainwide neuroanatomical connectivity in model organisms at a mesoscopic scale

Bohland, Jason and Wu, Caizhi and Barbas, Helen and Bokil, Hemant and Bota, Mihail and Breiter, Hans C. and Cline, Hollis T. and Doyle, John C. and Freed, Peter J. and Greenspan, Ralph J. and Haber, Suzanne N. and Hawrylycz, Michael and Herrera, Daniel G. and Hilgetag, Claus C. and Huang, Z. Josh and Jones, Allan and Jones, Edward G. and Karten, Harvey J. and Kleinfeld, David and Kötter, Rolf and Lester, Henry A. and Lin, John M. and Mensh, Brett D. and Mikula, Shawn and Panskepp, Jaak and Price, Joseph L. and Safdieh, Joseph and Saper, Clifford B. and Schiff, Nicholas D. and Schmahmann, Jeremy D. and Stillman, Bruce W. and Svoboda, Karel and Swanson, Larry W. and Toga, Arthur W. and Van Essen, David C. and Watson, James D. and Mitra, Partha P. (2009) A proposal for a coordinated effort for the determination of brainwide neuroanatomical connectivity in model organisms at a mesoscopic scale. PLoS Computational Biology, 5 (3). Art. No. e1000334. ISSN 1553-734X. PMCID PMC2655718. doi:10.1371/journal.pcbi.1000334.

PDF - Published Version
Creative Commons Attribution.


Use this Persistent URL to link to this item:


In this era of complete genomes, our knowledge of neuroanatomical circuitry remains surprisingly sparse. Such knowledge is critical, however, for both basic and clinical research into brain function. Here we advocate for a concerted effort to fill this gap, through systematic, experimental mapping of neural circuits at a mesoscopic scale of resolution suitable for comprehensive, brainwide coverage, using injections of tracers or viral vectors. We detail the scientific and medical rationale and briefly review existing knowledge and experimental techniques. We define a set of desiderata, including brainwide coverage; validated and extensible experimental techniques suitable for standardization and automation; centralized, open-access data repository; compatibility with existing resources; and tractability with current informatics technology. We discuss a hypothetical but tractable plan for mouse, additional efforts for the macaque, and technique development for human. We estimate that the mouse connectivity project could be completed within five years with a comparatively modest budget.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Doyle, John C.0000-0002-1828-2486
Greenspan, Ralph J.0000-0002-6787-2845
Lester, Henry A.0000-0002-5470-5255
Van Essen, David C.0000-0001-7044-4721
Additional Information:© 2009 Bohland et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published March 27, 2009. This paper is a result of discussions at the 2007 and 2008 Brain Architecture Project Banbury Center Meetings, funded by the W. M. Keck Foundation. The sponsors had no role in the conception or preparation of this manuscript.
Issue or Number:3
PubMed Central ID:PMC2655718
Record Number:CaltechAUTHORS:20090717-142050047
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:14612
Deposited On:24 Jul 2009 18:55
Last Modified:08 Nov 2021 23:13

Repository Staff Only: item control page