CaltechAUTHORS
  A Caltech Library Service

Cloning and sequencing of V-ATPase subunit d from mung bean and its function in passive proton transport

Ouyang, Zhuqing and Li, Zhuo and Zhang, Xujia (2008) Cloning and sequencing of V-ATPase subunit d from mung bean and its function in passive proton transport. Journal of Bioenergetics and Biomembranes, 40 (6). pp. 569-576. ISSN 0145-479X. http://resolver.caltech.edu/CaltechAUTHORS:20090722-153626905

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20090722-153626905

Abstract

We have previously shown that vacuolar H+-ATPase subcomplex V_o from mung bean contains subunit d, however, its sequence and function were unknown. In the present study, we report the cloning and recombinant over expression of subunit d from mung bean in E. coli. To study the function of subunit d, two vacuolar H+-ATPase subcomplexes V_o from mung bean were purified-one containing subunits a and c(c’,c”) and the other containing subunits a, c(c’,c”) and d. After reconstitution of the purified V_o subcomplexes into liposomes, the proton translocation was studied. Our results show that the V_o subcomplex in the absence of subunit d is a passive proton channel, while the V_o subcomplex in the presence of the subunit d is not. Taken together, our data supports the conclusion that the subunit d of the plant vacuolar H+-ATPase from mung bean is positioned at the central stalk and involved in the proton translocation across the tonoplast membrane.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1007/s10863-008-9193-3DOIArticle
https://link.springer.com/article/10.1007%2Fs10863-008-9193-3PublisherArticle
http://rdcu.be/wxg5PublisherFree ReadCube Access
Additional Information:©2008 Springer Science. Received: 29 October 2008 / Accepted: 15 December 2008 / Published online: 5 February 2009. We thank Dr. So Iwata (Imperical College London, UK) for provision of subunit C of Thermus thermophilus V-ATPase, Dr. Fei Sun (Institute of Biophysics, Chinese Academy of Sciences, Beijing, China) for help in data processing, Ankita Roy (National Institute of Environmental Health Sciences, NIH, USA) and Elitza Tocheva (California Institute of Technology, Pasadena, USA) for reading of the manuscript. This work was supported by National Basic Research Program of China (2004CB720000 and 2006CB911001) and National Natural Science Foundation of China (30770492).
Funders:
Funding AgencyGrant Number
National Basic Research Program of China2004CB720000
National Basic Research Program of China2006CB911001
National Natural Science Foundation of China30770492
Subject Keywords:Passive proton transport . cDNA sequence . Vacuolar H+-ATPase . Vigna . RACE-PCR . Liposome . Fluorescence quench . d subunit . Central stalk
Record Number:CaltechAUTHORS:20090722-153626905
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20090722-153626905
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:14645
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:03 Aug 2009 18:13
Last Modified:06 Oct 2017 17:31

Repository Staff Only: item control page