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Evidence of a mate-finding cue in the hermaphrodite nematode Caenorhabditis elegans

Simon, Jasper M. and Sternberg, Paul W. (2002) Evidence of a mate-finding cue in the hermaphrodite nematode Caenorhabditis elegans. Proceedings of the National Academy of Sciences of the United States of America, 99 (3). pp. 1598-1603. ISSN 0027-8424. PMCID PMC122236. http://resolver.caltech.edu/CaltechAUTHORS:SIMpnas02

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Abstract

When males of the roundworm Caenorhabditis elegans come into association with their hermaphroditic counterparts they cease foraging behavior and begin to mate. Here we detail several assays used to demonstrate that a diffusible cue is correlated with this process. This cue is sexually dimorphic, given off only by the hermaphrodite and eliciting a response only in the male. Males are attracted to, reverse direction of movement frequently, and remain in regions of agar conditioned with hermaphrodites. From our studies we suggest a form of kinesis that works by attracting males to their mating partners from a distance and functions, once males arrive, in holding attracted males in close proximity. The hermaphrodite vulva is not required for the cue. Males from general sensory mutants osm-5 and osm-6 fail to respond to the cue, whereas male-specific mutants lov-1 and pkd-2 respond. Finally, that males from multiple isolates of C elegans also respond similarly to this cue indicates that this cue is robust and has been maintained during recent evolution.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC122236/PubMed CentralArticle
ORCID:
AuthorORCID
Sternberg, Paul W.0000-0002-7699-0173
Additional Information:© 2002 by the National Academy of Sciences. Edited by H. Robert Horvitz, Massachusetts Institute of Technology, Cambridge, MA, and approved December 4, 2001 (received for review May 7, 2001). Published online before print January 29, 2002, 10.1073/pnas.032225799. We thank R. Garcia and N. Moghal for discussions, J. DeModena for experimental suggestions, S. Mukhtar for setting up the video-tracking system, and members of the Sternberg and Benzer laboratories for experimental guidance and reading drafts of our manuscript. The Caenorhabditis Genetics Center provided strains. This work was supported by the Howard Hughes Medical Institute, with which P.W.S. is an investigator. This paper was submitted directly (Track II) to the PNAS office. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Subject Keywords:SENSORY BEHAVIOR; MATING BEHAVIOR; KINESIS; MALE MATING-BEHAVIOR; DROSOPHILA-MELANOGASTER; NATURAL VARIATION; CELL LINEAGES; C-ELEGANS; PHEROMONES; OLFACTION; RESPONSES; MUTANTS; ODOR
PubMed Central ID:PMC122236
Record Number:CaltechAUTHORS:SIMpnas02
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:SIMpnas02
Alternative URL:http://dx.doi.org/10.1073/pnas.032225799
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1470
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:21 Jan 2006
Last Modified:09 May 2019 23:48

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