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Iterative in situ click chemistry creates antibody-like protein-capture agents

Agnew, Heather D. and Rohde, Rosemary D. and Millward, Steven W. and Nag, Arundhati and Yeo, Woon-Seok and Hein, Jason E. and Pitram, Suresh M. and Tariq, Abdul Ahad and Burns, Vanessa M. and Krom, Russell J. and Fokin, Valery V. and Sharpless, K. Barry and Heath, James R. (2009) Iterative in situ click chemistry creates antibody-like protein-capture agents. Angewandte Chemie International Edition, 48 (27). pp. 4944-4948. ISSN 1433-7851. PMCID PMC3716464.

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Iterative in situ click chemistry (see scheme for the tertiary ligand screen) and the one-bead-one-compound method for the creation of a peptide library enable the fragment-based assembly of selective high-affinity protein-capture agents. The resulting ligands are water-soluble and stable chemically, biochemically, and thermally. They can be produced in gram quantities through copper (I)-catalyzed cycloaddition.

Item Type:Article
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URLURL TypeDescription CentralArticle
Sharpless, K. Barry0000-0001-6051-1599
Heath, James R.0000-0001-5356-4385
Additional Information:© 2009 Wiley VCH Verlag GmbH & Co. KGaA, Weinheim. Received: January 26, 2009. Published online: March 19, 2009. This research was supported by the National Cancer Institute, grant No. 5U54 CA119347 (J.R.H.), the W. M. Keck Foundation (K.B.S.), and a subcontract from the MITRE Corporation (J.R.H.). Postdoctoral and graduate fellowships were provided by the NSF and PEO (H.D.A.), the Gates Foundation (R.D.R.), the NSERC (J.E.H.), and the NCI (S.W.M.). We also acknowledge H. C. Kolb, S. S. Lee, J. Cha, J. Lim, S. Tan, S. Y. Yeo, A. Srivastava, M. Barrientos, E. Johnson Chavarria, A. Stuparu, J. Vielmetter, and C. S. Parker for useful discussions and assistance.
Funding AgencyGrant Number
National Cancer Institute5U54 CA119347
W. M. Keck FoundationUNSPECIFIED
Bill and Melinda Gates FoundationUNSPECIFIED
Natural Sciences and Engineering Research Council of Canada (NSERC)UNSPECIFIED
Subject Keywords:click chemistry, combinatorial chemistry, inhibitors, peptides, protein capture agents
Issue or Number:27
PubMed Central ID:PMC3716464
Record Number:CaltechAUTHORS:20090804-133827150
Persistent URL:
Official Citation:Agnew, H., Rohde, R., Millward, S., Nag, A., Yeo, W.‐S., Hein, J., Pitram, S., Tariq, A., Burns, V., Krom, R., Fokin, V., Sharpless, K. and Heath, J. (2009), Iterative In Situ Click Chemistry Creates Antibody‐like Protein‐Capture Agents. Angewandte Chemie International Edition, 48: 4944-4948. doi:10.1002/anie.200900488
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:14792
Deposited By: Ruth Sustaita
Deposited On:06 Aug 2009 23:10
Last Modified:23 Dec 2019 17:13

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