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Physical association of ubiquitin ligases and the 26S proteasome

Xie, Youming and Varshavsky, Alexander (2000) Physical association of ubiquitin ligases and the 26S proteasome. Proceedings of the National Academy of Sciences of the United States of America, 97 (6). pp. 2497-2502. ISSN 0027-8424. PMCID PMC15957. https://resolver.caltech.edu/CaltechAUTHORS:XIEpnas00

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Abstract

The ubiquitin (Ub) system recognizes degradation signals of the target proteins through the E3 components of E3-E2 Ub ligases. A targeted substrate bears a covalently linked multi-Ub chain and is degraded by the ATP-dependent 26S proteasome, which consists of the 20S core protease and two 19S particles. The latter mediate the binding and unfolding of a substrate protein before its transfer to the interior of the 20S core. It is unclear how a targeted substrate is delivered to the 26S proteasome, inasmuch as Rpn10p, the only known proteasomal subunit that binds multi-Ub chains, has been found to he not essential for degradation of many proteins in the yeast Saccharomyces cerevisiae. Here we show that Ubr1p and Ufd4p, the E3 components of two distinct Ub ligases, directly interact with the 26S proteasome. Specifically, Ubr1p is shown to bind to the Rpn2p, Rpt1p, and Rpt6p proteins of the 19S particle, and Ufd4p is shown to bind to Rpt6p. These and related results suggest that a substrate-bound Ub ligase participates in the delivery of substrates to the proteasome, because of affinity between the ligase's E3 component and specific proteins of the 19S particle.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC15957/PubMed CentralArticle
https://doi.org/10.1073/pnas.060025497DOIUNSPECIFIED
https://doi.org/10.1073/pnas.060025497DOIUNSPECIFIED
ORCID:
AuthorORCID
Varshavsky, Alexander0000-0002-4011-258X
Additional Information:© 2000 by the National Academy of Sciences. Contributed by Alexander Varshavsky, January 19, 2000. Published online before print February 25, 2000, 10.1073/pnas.060025497. We thank F. Du and A. Webster for the gifts of purified FLAG-Ubr1p and the UPR-based plasmids, C. Pickart for isolated multi-Ub chains, H. Rao for the strain AVY107, and E. Johnson for the pRS313UFD4 plasmid. This work was supported by grants to A.V. from the National Institutes of Health (GM31530 and DK39520). Y.X. was supported in part by a postdoctoral fellowship from the National Institutes of Health. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
NIHGM31530
NIHDK39520
NIH Postdoctoral FellowshipUNSPECIFIED
Subject Keywords:E3; N-end rule; ubiquitin fusion degradation pathway; Ubr1p; Ufd4p
Issue or Number:6
PubMed Central ID:PMC15957
Record Number:CaltechAUTHORS:XIEpnas00
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:XIEpnas00
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1488
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Jan 2006
Last Modified:02 Oct 2019 22:43

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