Visualization of RNA tertiary struture by RNA-EDTA·Fe(II) autocleavage: Analysis of tRNAPhe with uridine-EDTA·Fe(II) at position 47

Han, Hogyu and Dervan, Peter B. (1994) Visualization of RNA tertiary struture by RNA-EDTA·Fe(II) autocleavage: Analysis of tRNAPhe with uridine-EDTA·Fe(II) at position 47. Proceedings of the National Academy of Sciences of the United States of America, 91 (11). pp. 4955-4959. ISSN 0027-8424. PMCID PMC43908. https://resolver.caltech.edu/CaltechAUTHORS:HANpnas94

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Abstract

To test whether intramolecular autocleavage of RNA labeled at a single nucleotide position with EDTA.Fe(II) is a useful approach for the investigation of tertiary structures of RNAs, yeast phenylalanine tRNA was synthesized with uridine-EDTA (*U) at position U47. Autocleavage of [*U47]tRNA(Phe) in the presence of Fe(NH4)(2)(SO4)(2) and dithiothreitol produced a set of cleavage fragments which are in general agreement with the three-dimensional structure derived from x-ray analysis.

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Article

Related URLs:

URL	URL Type	Description
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC43908/	PubMed Central	Article
http://www.pnas.org/cgi/content/abstract/91/11/4955	Other	UNSPECIFIED
http://www.pnas.org/cgi/content/abstract/91/11/4955	Other	UNSPECIFIED

ORCID:

Author	ORCID
Dervan, Peter B.	0000-0001-8852-7306

Additional Information:

© 1994 by the National Academy of Sciences. Contributed by Peter B. Dervan, February 24, 1993. We are grateful to the National Institutes of Health for grant support. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

Funders:

Funding Agency	Grant Number
NIH	UNSPECIFIED

Subject Keywords:

AFFINITY CLEAVING; YEAST TRNA(PHE); CLEAVAGE OF RNA; PHENYLALANINE TRANSFER-RNA; SEQUENCE-SPECIFIC CLEAVAGE; YEAST TRANSFER-RNAPHE; TRIPLE-HELIX FORMATION; BINDING-SITES; DNA; MOLECULES; RECOGNITION; RESOLUTION; SYNTHETASE

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PubMed Central ID:

PMC43908

Record Number:

CaltechAUTHORS:HANpnas94

Persistent URL:

https://resolver.caltech.edu/CaltechAUTHORS:HANpnas94

Official Citation:

H Han and P B Dervan Visualization of RNA tertiary structure by RNA-EDTA.Fe(II) autocleavage: analysis of tRNA(Phe) with uridine-EDTA.Fe(II) at position 47 PNAS 1994 91 (11) 4955-4959

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ID Code:

1500

Collection:

CaltechAUTHORS

Deposited By:

Tony Diaz

Deposited On:

26 Jan 2006

Last Modified:

26 Nov 2019 11:15

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