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Atypical pattern of utilization of amino acids for mitochondrial protein synthesis in HeLa cells

Costantino, Paolo and Attardi, Giuseppe (1973) Atypical pattern of utilization of amino acids for mitochondrial protein synthesis in HeLa cells. Proceedings of the National Academy of Sciences of the United States of America, 70 (5). pp. 1490-1494. ISSN 0027-8424.

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The capacity of HeLa cell mitochondria, either isolated or in intact cells, to incorporate different labeled amino acids into proteins was investigated. Eight amino acids (alanine, arginine, aspartic acid, cysteine, glutamic acid, glutamine, glycine, and lysine), which include most of the charged polar ones, showed a very low amount, if any at all, of chloramphenicol-sensitive incorporation, relative to that expected for an "average" HeLa-cell protein. By contrast, the most hydrophobic amino acids (leucine, isoleucine, valine, phenylalanine, and methionine) were the most actively incorporated by HeLa mitochondria. The available evidence suggests that pool effects cannot account for this general pattern of utilization of amino acids; furthermore, this pattern is in good agreement with the known hydrophobic properties of proteins synthesized in mitochondria.

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Additional Information:Copyright © 1973 by the National Academy of Sciences. Communicated by Norman Davidson, February 26, 1973. These investigations were supported by a research grant from the U.S. Public Health Service (GM-11726). The help of Mrs. B. Keeley and Miss G. Engel is gratefully acknowledged.
Subject Keywords:chloramphenicol; endoplasmic reticulum; amino-acid pools; mitochondrial tRNA
Issue or Number:5
Record Number:CaltechAUTHORS:COSpnas73
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1508
Deposited By: Tony Diaz
Deposited On:25 Jan 2006
Last Modified:02 Oct 2019 22:44

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