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Fluorescein Redirects a Ruthenium−Octaarginine Conjugate to the Nucleus

Puckett, Cindy A. and Barton, Jacqueline K. (2009) Fluorescein Redirects a Ruthenium−Octaarginine Conjugate to the Nucleus. Journal of the American Chemical Society, 131 (25). pp. 8738-8739. ISSN 0002-7863. PMCID PMC2747598. doi:10.1021/ja9025165. https://resolver.caltech.edu/CaltechAUTHORS:20090901-133357644

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Abstract

The cellular uptake and localization of a Ru−octaarginine conjugate with and without an appended fluorescein are compared. The inherent luminescence of the Ru(II) dipyridophenazine complex allows observation of its uptake without the addition of a fluorophore. Ru−octaarginine−fluorescein stains the cytosol, nuclei, and nucleoli of HeLa cells under conditions where the Ru−octaarginine conjugate without fluorescein shows only punctate cytoplasmic labeling. At higher concentrations, however, Ru−octaarginine without the fluorescein tag does exhibit cytoplasmic, nuclear, and nucleolar staining. Attaching fluorescein to Ru−octaarginine lowers the threshold concentration required for diffuse cytoplasmic labeling and nuclear entry. Hence, the localization of the fluorophore-bound peptide cannot serve as a proxy for that of the free peptide.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja9025165DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja9025165PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2747598/PubMed CentralArticle
ORCID:
AuthorORCID
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:Copyright © 2009 American Chemical Society. Received March 30, 2009; Publication Date (Web): June 8, 2009. We are grateful to the NIH (GM33309) and to the Caltech Biological Imaging Center.
Funders:
Funding AgencyGrant Number
NIHGM33309
Issue or Number:25
PubMed Central ID:PMC2747598
DOI:10.1021/ja9025165
Record Number:CaltechAUTHORS:20090901-133357644
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20090901-133357644
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:15527
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:09 Sep 2009 22:53
Last Modified:08 Nov 2021 23:20

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