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Directed enzyme evolution: climbing fitness peaks one amino acid at a time

Tracewell, Cara A. and Arnold, Frances H. (2009) Directed enzyme evolution: climbing fitness peaks one amino acid at a time. Current Opinion in Chemical Biology, 13 (1). pp. 3-9. ISSN 1367-5931. PMCID PMC2703427. doi:10.1016/j.cbpa.2009.01.017. https://resolver.caltech.edu/CaltechAUTHORS:20090910-084155263

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Abstract

Directed evolution can generate a remarkable range of new enzyme properties. Alternate substrate specificities and reaction selectivities are readily accessible in enzymes from families that are naturally functionally diverse. Activities on new substrates can be obtained by improving variants with broadened specificities or by step-wise evolution through a sequence of more and more challenging substrates. Evolution of highly specific enzymes has been demonstrated, even with positive selection alone. It is apparent that many solutions exist for any given problem, and there are often many paths that lead uphill, one step at a time.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.cbpa.2009.01.017DOIArticle
http://www.sciencedirect.com/science/article/pii/S1367593109000076PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2703427PubMed CentralArticle
ORCID:
AuthorORCID
Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2009 Elsevier. Available online 25 February 2009. CAT was supported by Ruth M Kirschstein National Research Service Award F32 GM076964 and NIH grant R01 GM074712-01A1. We also acknowledge support from the Institute for Collaborative Biotechnologies, the Jacobs Institute for Molecular Medicine, and the Department of Energy.
Funders:
Funding AgencyGrant Number
NIH Predoctoral FellowshipF32 GM076964
NIHR01 GM074712-01A1
Army Research Office (ARO)UNSPECIFIED
Jacobs Institute for Molecular MedicineUNSPECIFIED
Department of Energy (DOE)UNSPECIFIED
Issue or Number:1
PubMed Central ID:PMC2703427
DOI:10.1016/j.cbpa.2009.01.017
Record Number:CaltechAUTHORS:20090910-084155263
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20090910-084155263
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:15711
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:10 Sep 2009 21:11
Last Modified:08 Nov 2021 23:21

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