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^1H{^(19)F} NOE NMR Structural Signatures of the Insulin R_6 Hexamer: Evidence of a Capped HisB10 Site in Aryl- and Arylacryloyl-carboxylate Complexes

Keidel, Donald and Bonaccio, Maria and Ghaderi, Nima and Niks, Dimitri and Borchardt, Dan and Dunn, Michael F. (2009) ^1H{^(19)F} NOE NMR Structural Signatures of the Insulin R_6 Hexamer: Evidence of a Capped HisB10 Site in Aryl- and Arylacryloyl-carboxylate Complexes. ChemBioChem, 10 (3). pp. 450-453. ISSN 1439-4227. https://resolver.caltech.edu/CaltechAUTHORS:20090910-084752456

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Abstract

New and improved insulin: ^1H{^(19)F} NOE NMR difference spectra for CF_3-substituted aromatic carboxylates bound at the HisB10 sites of the R_6 human insulin (HI) hexamer show strong NOEs between the CF_3 groups and the LeuB6, AsnB3, and PheB1 sidechains. The NOEs and structural modeling establish that these carboxylates form closed complexes with the HisB10 site capped by the PheB1 rings.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/cbic.200800746DOIUNSPECIFIED
http://www3.interscience.wiley.com/cgi-bin/fulltext/121638384/HTMLSTARTPublisherUNSPECIFIED
ORCID:
AuthorORCID
Ghaderi, Nima0000-0002-6257-1373
Additional Information:© 2009 WILEY-VCH Verlag GmbH & Co. Received: November 12, 2008 Published online on January 14, 2009. We thank Novo Nordisk for the wild-type human and variant insulin, and for a gift to M.F.D. in support of this work.
Funders:
Funding AgencyGrant Number
Novo NordiskUNSPECIFIED
Subject Keywords:^(19)F NMR; allosterism; insulin; molecular modeling; NMR spectroscopy; nuclear Overhauser effect
Issue or Number:3
Record Number:CaltechAUTHORS:20090910-084752456
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20090910-084752456
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:15713
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:10 Sep 2009 17:54
Last Modified:09 Mar 2020 13:18

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