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High-Potential C112D/M121X (X = M, E, H, L) Pseudomonas aeruginosa Azurins

Lancaster, Kyle M. and Yokoyama, Keiko and Richards, John H. and Winkler, Jay R. and Gray, Harry B. (2009) High-Potential C112D/M121X (X = M, E, H, L) Pseudomonas aeruginosa Azurins. Inorganic Chemistry, 48 (4). pp. 1278-1280. ISSN 0020-1669. PMCID PMC2765487. doi:10.1021/ic802322e.

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Site-directed mutagenesis of Pseudomonas aeruginosa azurin C112D at the M121 position has afforded a series of proteins with elevated Cu^(II/I) reduction potentials relative to the CuII aquo ion. The high potential and low axial hyperfine splitting (Cu^(II) electron paramagnetic resonance A|) of the C112D/M121L protein are remarkably similar to features normally associated with type 1 copper centers.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Lancaster, Kyle M.0000-0001-7296-128X
Winkler, Jay R.0000-0002-4453-9716
Gray, Harry B.0000-0002-7937-7876
Additional Information:© 2009 American Chemical Society. Received December 3, 2008. We thank Yuling Sheng and Matthew R. Hartings for assistance and Israel Pecht for useful discussions. This work was supported by the NSF (Grant CHE-0553150 to H.B.G. and J.R.W.; graduate fellowship to K.M.L.), GCEP (Stanford), and NIH (Grant DK019038 to H.B.G.)
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Issue or Number:4
PubMed Central ID:PMC2765487
Record Number:CaltechAUTHORS:20090910-101427356
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Official Citation:High-Potential C112D/M121X (X = M, E, H, L) Pseudomonas aeruginosa Azurins Kyle M. Lancaster, Keiko Yokoyama, John H. Richards, Jay R. Winkler, Harry B. Gray Inorganic Chemistry 2009 48 (4), 1278-1280
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:15723
Deposited By: Ruth Sustaita
Deposited On:10 Sep 2009 17:36
Last Modified:08 Nov 2021 23:21

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