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Comparative Analysis of Methane-Oxidizing Archaea and Sulfate-Reducing Bacteria in Anoxic Marine Sediments

Orphan, V. J. and Hinrichs, K.-U. and Ussler, W., III and Paull, C. K. and Taylor, L. T. and Sylva, S. P. and Hayes, J. M. and DeLong, E. F. (2001) Comparative Analysis of Methane-Oxidizing Archaea and Sulfate-Reducing Bacteria in Anoxic Marine Sediments. Applied and Environmental Microbiology, 67 (4). pp. 1922-1934. ISSN 0099-2240. PMCID PMC92814. https://resolver.caltech.edu/CaltechAUTHORS:ORPaem01

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Abstract

The oxidation of methane in anoxic marine sediments is thought to be mediated by a consortium of methane-consuming archaea and sulfate-reducing bacteria. In this study, we compared results of rRNA gene (rDNA) surveys and lipid analyses of archaea and bacteria associated with methane seep sediments from several different sites on the Californian continental margin. Two distinct archaeal lineages (ANME-1 and ANME-2), peripherally related to the order Methanosarcinales, were consistently associated with methane seep marine sediments. The same sediments contained abundant 13C-depleted archaeal lipids, indicating that one or both of these archaeal groups are members of anaerobic methane-oxidizing consortia. 13C-depleted lipids and the signature 16S rDNAs for these archaeal groups were absent in nearby control sediments. Concurrent surveys of bacterial rDNAs revealed a predominance of delta -proteobacteria, in particular, close relatives of Desulfosarcina variabilis. Biomarker analyses of the same sediments showed bacterial fatty acids with strong 13C depletion that are likely products of these sulfate-reducing bacteria. Consistent with these observations, whole-cell fluorescent in situ hybridization revealed aggregations of ANME-2 archaea and sulfate-reducing Desulfosarcina and Desulfococcus species. Additionally, the presence of abundant 13C-depleted ether lipids, presumed to be of bacterial origin but unrelated to ether lipids of members of the order Desulfosarcinales, suggests the participation of additional bacterial groups in the methane-oxidizing process. Although the Desulfosarcinales and ANME-2 consortia appear to participate in the anaerobic oxidation of methane in marine sediments, our data suggest that other bacteria and archaea are also involved in methane oxidation in these environments.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC92814/PubMed CentralArticle
ORCID:
AuthorORCID
Orphan, V. J.0000-0002-5374-6178
Additional Information:Copyright © 2001, American Society for Microbiology. Received 10 October 2000/Accepted 2 February 2001. Funding for this project was provided by the David and Lucile Packard Foundation and a NASA isotopic biogeochemistry grant, NAG5-9422, to J.M.H. K.-U.H. thanks the Hanse Institute of Advanced Study in Delmenhorst, Germany, for a fellowship, during which the manuscript was completed. We thank Andreas Teske, Jon Martin, Jim Barry, and Thomas Naehr for graciously supplying data used in this study. We also thank Shana Goffredi for helpful comments on the manuscript; Josh Plant, Christopher Lovera, and the crew of the R.V. Point Lobos for their invaluable assistance in sample collection and processing; and A. Boetius and D. Valentine for sharing their unpublished manuscripts with us.
Funders:
Funding AgencyGrant Number
David and Lucile Packard FoundationUNSPECIFIED
NASANAG5-9422
Hanse Institute of Advanced StudyUNSPECIFIED
Issue or Number:4
PubMed Central ID:PMC92814
Record Number:CaltechAUTHORS:ORPaem01
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:ORPaem01
Alternative URL:http://dx.doi.org/10.1128/AEM.67.4.1922-1934.2001
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1579
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:31 Jan 2006
Last Modified:02 Oct 2019 22:44

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