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Model for eukaryotic tail-anchored protein binding based on the structure of Get3

Suloway, Christian J. M. and Chartron, Justin W. and Zaslaver, Ma'ayan and Clemons, William M., Jr. (2009) Model for eukaryotic tail-anchored protein binding based on the structure of Get3. Proceedings of the National Academy of Sciences of the United States of America, 106 (35). pp. 14849-14854. ISSN 0027-8424. PMCID PMC2736419.

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The Get3 ATPase directs the delivery of tail-anchored (TA) proteins to the endoplasmic reticulum (ER). TA-proteins are characterized by having a single transmembrane helix (TM) at their extreme C terminus and include many essential proteins, such as SNAREs, apoptosis factors, and protein translocation components. These proteins cannot follow the SRP-dependent co-translational pathway that typifies most integral membrane proteins; instead, post-translationally, these proteins are recognized and bound by Get3 then delivered to the ER in the ATP dependent Get pathway. To elucidate a molecular mechanism for TA protein binding by Get3 we have determined three crystal structures in apo and ADP forms from Saccharomyces cerevisae (ScGet3-apo) and Aspergillus fumigatus (AfGet3-apo and AfGet3-ADP). Using structural information, we generated mutants to confirm important interfaces and essential residues. These results point to a model of how Get3 couples ATP hydrolysis to the binding and release of TA-proteins.

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URLURL TypeDescription CentralArticle Information
Clemons, William M., Jr.0000-0002-0021-889X
Additional Information:©2009 by the National Academy of Sciences. Communicated by Douglas C. Rees, California Institute of Technology, Pasadena, CA, July 8, 2009 (received for review June 24, 2009). Published online before print August 14, 2009, doi: 10.1073/pnas.0907522106 We thank S. Shan and A. Palazzo for discussion and critical comments on the manuscript and Gordon and Betty Moore for support of the Molecular Observatory at Caltech. All data collection was performed at beamline 12-2 at Stanford Synchrotron Radiation Lightsource and we thank G. Card and M. Soltis for beamline assistance. Operations at SSRL are supported by the U.S. Department of Energy and National Institutes of Health. W.M.C. is supported by the Searle Scholar program and a Burroughs-Wellcome Fund Career Award for the Biological Sciences. Author contributions: C.J.M.S. and W.M.C. designed research; C.J.M.S. performed research; C.J.M.S. and M.Z. contributed new reagents/analytic tools; C.J.M.S., J.W.C., and W.M.C. analyzed data; and C.J.M.S., J.W.C., and W.M.C. wrote the paper. The authors declare no conflict of interest. Data deposition: The atomic coordinates and structure factors have been deposited in the RCSB Protein Data Bank, (PDB ID codes 31BG for AfGet3-ADP and 31DQ for ScGet3). This article contains supporting information online at
Funding AgencyGrant Number
Gordon and Betty Moore FoundationUNSPECIFIED
Department of Energy (DOE)UNSPECIFIED
Searle Scholars ProgramUNSPECIFIED
Burroughs-Wellcome FundUNSPECIFIED
Subject Keywords:ArsA; crystallography; Deviant Walker A; Get pathway; protein transport
Issue or Number:35
PubMed Central ID:PMC2736419
Record Number:CaltechAUTHORS:20090911-153558382
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:15790
Deposited By: George Porter
Deposited On:02 Oct 2009 18:44
Last Modified:03 Oct 2019 01:02

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