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A Distinct Mechanism to Achieve Efficient Signal Recognition Particle (SRP)-SRP Receptor Interaction by the Chloroplast SRP Pathway

Jaru-Ampornpan, Peera and Nguyen, Thang X. and Shan, Shu-ou (2009) A Distinct Mechanism to Achieve Efficient Signal Recognition Particle (SRP)-SRP Receptor Interaction by the Chloroplast SRP Pathway. Molecular Biology of the Cell, 20 (17). pp. 3965-3973. ISSN 1059-1524. PMCID PMC2735494. https://resolver.caltech.edu/CaltechAUTHORS:20090911-153600633

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Abstract

Cotranslational protein targeting by the signal recognition particle (SRP) requires the SRP RNA, which accelerates the interaction between the SRP and SRP receptor 200-fold. This otherwise universally conserved SRP RNA is missing in the chloroplast SRP (cpSRP) pathway. Instead, the cpSRP and cpSRP receptor (cpFtsY) by themselves can interact 200-fold faster than their bacterial homologues. Here, cross-complementation analyses revealed the molecular origin underlying their efficient interaction. We found that cpFtsY is 5- to 10-fold more efficient than Escherichia coli FtsY at interacting with the GTPase domain of SRP from both chloroplast and bacteria, suggesting that cpFtsY is preorganized into a conformation more conducive to complex formation. Furthermore, the cargo-binding M-domain of cpSRP provides an additional 100-fold acceleration for the interaction between the chloroplast GTPases, functionally mimicking the effect of the SRP RNA in the cotranslational targeting pathway. The stimulatory effect of the SRP RNA or the M-domain of cpSRP is specific to the homologous SRP receptor in each pathway. These results strongly suggest that the M-domain of SRP actively communicates with the SRP and SR GTPases and that the cytosolic and chloroplast SRP pathways have evolved distinct molecular mechanisms (RNA vs. protein) to mediate this communication.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1091/mbc.E08-10-0989DOIArticle
http://www.molbiolcell.org/cgi/content/abstract/20/17/3965PublisherArticle
http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E08-10-0989PublisherSupplemental Material
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2735494/PubMed CentralArticle
ORCID:
AuthorORCID
Shan, Shu-ou0000-0002-6526-1733
Additional Information:Copyright © 2009 by The American Society for Cell Biology. Under the License and Publishing Agreement, authors grant to the general public, effective two months after publication of (i.e.,. the appearance of) the edited manuscript in an online issue of MBoC, the nonexclusive right to copy, distribute, or display the manuscript subject to the terms of the Creative Commons–Noncommercial–Share Alike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0). Submitted October 2, 2008; Revised June 23, 2009; Accepted June 25, 2009. This was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E08-10-0989) on July 8, 2009. We thank the members of the Shan laboratory for helpful comments on the manuscript. This work was supported by National Institutes of Health grant GM-078024 (to S. S.). S. S. was supported by the Burroughs Wellcome Fund career award, the Beckman Young Investigator award, and the Packard and Lucile award in science and engineering. P.J.-A. was supported by a fellowship from the Brey Endowment foundation.
Funders:
Funding AgencyGrant Number
NIHGM-078024
Burroughs Wellcome FundUNSPECIFIED
Arnold and Mabel Beckman FoundationUNSPECIFIED
David and Lucile Packard FoundationUNSPECIFIED
Brey Endowment FoundationUNSPECIFIED
Issue or Number:17
PubMed Central ID:PMC2735494
Record Number:CaltechAUTHORS:20090911-153600633
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20090911-153600633
Official Citation:Peera Jaru-Ampornpan, Thang X. Nguyen, and Shu-ou Shan A Distinct Mechanism to Achieve Efficient Signal Recognition Particle (SRP)–SRP Receptor Interaction by the Chloroplast SRP Pathway Mol. Biol. Cell 2009 20: 3965-3973; published online before print as 10.1091/mbc.E08-10-0989
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:15801
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:02 Oct 2009 20:59
Last Modified:03 Oct 2019 01:03

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