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Mutations in UDP-Glucose:Sterol Glucosyltransferase in Arabidopsis Cause Transparent Testa Phenotype and Suberization Defect in Seeds

DeBolt, Seth and Scheible, Wolf-Rüdiger and Schrick, Kathrin and Auer, Manfred and Beisson, Fred and Bischoff, Volker and Bouvier-Navé, Pierrette and Carroll, Andrew and Hematy, Kian and Li, Yonghua and Milne, Jennifer and Nair, Meera and Schaller, Hubert and Zemla, Marcin and Somerville, Chris (2009) Mutations in UDP-Glucose:Sterol Glucosyltransferase in Arabidopsis Cause Transparent Testa Phenotype and Suberization Defect in Seeds. Plant Physiology, 151 (1). pp. 78-87. ISSN 0032-0889. https://resolver.caltech.edu/CaltechAUTHORS:20090923-143133808

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Abstract

In higher plants, the most abundant sterol derivatives are steryl glycosides (SGs) and acyl SGs. Arabidopsis (Arabidopsis thaliana) contains two genes, UGT80A2 and UGT80B1, that encode UDP-Glc:sterol glycosyltransferases, enzymes that catalyze the synthesis of SGs. Lines having mutations in UGT80A2, UGT80B1, or both UGT80A2 and UGT8B1 were identified and characterized. The ugt80A2 lines were viable and exhibited relatively minor effects on plant growth. Conversely, ugt80B1 mutants displayed an array of phenotypes that were pronounced in the embryo and seed. Most notable was the finding that ugt80B1 was allelic to transparent testa15 and displayed a transparent testa phenotype and a reduction in seed size. In addition to the role of UGT80B1 in the deposition of flavanoids, a loss of suberization of the seed was apparent in ugt80B1 by the lack of autofluorescence at the hilum region. Moreover, in ugt80B1, scanning and transmission electron microscopy reveals that the outer integument of the seed coat lost the electron-dense cuticle layer at its surface and displayed altered cell morphology. Gas chromatography coupled with mass spectrometry of lipid polyester monomers confirmed a drastic decrease in aliphatic suberin and cutin-like polymers that was associated with an inability to limit tetrazolium salt uptake. The findings suggest a membrane function for SGs and acyl SGs in trafficking of lipid polyester precursors. An ancillary observation was that cellulose biosynthesis was unaffected in the double mutant, inconsistent with a predicted role for SGs in priming cellulose synthesis.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1104/pp.109.140582DOIUNSPECIFIED
http://www.plantphysiol.org/cgi/content/abstract/151/1/78PublisherUNSPECIFIED
Additional Information:Copyright © 2009 by the American Society of Plant Biologists. Open access articles can be viewed online without a subscription. Received April 29, 2009; accepted July 20, 2009; published July 29, 2009. We thank Elliot Meyerowitz (California Institute of Technology), Cindy Cordova, Grace Qi (Keck Graduate Institute), and Darby Harris (University of Kentucky) for technical assistance, and Dirk Warneke (University of Hamburg) and Chris Shaw (University of British Columbia) for helpful discussion. This work was supported by grants from the Balzan Foundation and the U.S. Department of Energy (grant no. DE–FG02–09ER16008 to C.S. and grant no. NSF:IOS–0922947 to S.D.). K.S. was supported by the U.S. Department of Agriculture (grant no. USDA:2007–35304–18453) and the National Science Foundation (grant no. NSF:MCB–051778). The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Seth DeBolt (sdebo2@email.uky.edu). Some figures in this article are displayed in color online but in black and white in the print edition. The online version of this article contains Web-only data.
Funders:
Funding AgencyGrant Number
Balzan FoundationUNSPECIFIED
Department of EnergyDE–FG02–09ER16008
NSFIOS–0922947
Department of Agriculture2007–35304–18453
NSFMCB–051778
Issue or Number:1
Record Number:CaltechAUTHORS:20090923-143133808
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20090923-143133808
Official Citation:Seth DeBolt, Wolf-Rüdiger Scheible, Kathrin Schrick, Manfred Auer, Fred Beisson, Volker Bischoff, Pierrette Bouvier-Navé, Andrew Carroll, Kian Hematy, Yonghua Li, Jennifer Milne, Meera Nair, Hubert Schaller, Marcin Zemla, and Chris Somerville Mutations in UDP-Glucose:Sterol Glucosyltransferase in Arabidopsis Cause Transparent Testa Phenotype and Suberization Defect in Seeds Plant Physiol. 151: 78-87; Plant Physiol. First published on September 1, 2009; 10.1104/pp.109.140582
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:16012
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:07 Oct 2009 20:09
Last Modified:03 Oct 2019 01:06

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