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Extensive carbon isotopic heterogeneity among methane seep microbiota

House, Christopher H. and Orphan, Victoria J. and Turk, Kendra A. and Thomas, Burt and Pernthaler, Annelie and Vrentas, Jennifer M. and Joye, Samantha B. (2009) Extensive carbon isotopic heterogeneity among methane seep microbiota. Environmental Microbiology, 11 (9). pp. 2207-2215. ISSN 1462-2912. https://resolver.caltech.edu/CaltechAUTHORS:20090923-143137088

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Abstract

To assess and study the heterogeneity of δ^(13)C values for seep microorganisms of the Eel River Basin, we studied two principally different sample sets: sediments from push cores and artificial surfaces colonized over a 14 month in situ incubation. In a single sediment core, the δ^(13)C compositions of methane seep-associated microorganisms were measured and the relative activity of several metabolisms was determined using radiotracers. We observed a large range of archaeal δ^(13)C values (> 50‰) in this microbial community. The δ^(13)C of ANME-1 rods ranged from −24‰ to −87‰. The δ^(13)C of ANME-2 sarcina ranged from −18‰ to −75‰. Initial measurements of shell aggregates were as heavy as −19.5‰ with none observed to be lighter than −57‰. Subsequent measurements on shell aggregates trended lighter reaching values as ^(13)C-depleted as −73‰. The observed isotopic trends found for mixed aggregates were similar to those found for shell aggregates in that the initial measurements were often enriched and the subsequent analyses were more ^(13)C-depleted (with values as light as −56‰). The isotopic heterogeneity and trends observed within taxonomic groups suggest that ANME-1 and ANME-2 sarcina are capable of both methanogenesis and methanotrophy. In situ microbial growth was investigated by incubating a series of slides and silicon (Si) wafers for 14 months in seep sediment. The experiment showed ubiquitous growth of bacterial filaments (mean δ^(13)C = −38 ± 3‰), suggesting that this bacterial morphotype was capable of rapid colonization and growth.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1111/j.1462-2920.2009.01934.xDOIArticle
http://www3.interscience.wiley.com/journal/122443375/abstract?CRETRY=1&SRETRY=0PublisherArticle
ORCID:
AuthorORCID
House, Christopher H.0000-0002-4926-4985
Orphan, Victoria J.0000-0002-5374-6178
Joye, Samantha B.0000-0003-1610-451X
Additional Information:Copyright © 2009 Society for Applied Microbiology and Blackwell Publishing Ltd. Received 25 November, 2008; accepted 11 March, 2009. Published Online: 7 June 2009. We thank Zhidan Zhang and Tsegereda Embaye for laboratory assistance, and the captain and crew of the R/V Western Flyer and ROV Tiburon for their tireless efforts during the field expedition. We also thank Katherine H. Freeman for the opportunity to measure the δ^(13)C isotopic composition of our Escherichia coli cells by EA-CF-IRMS in the PSU Isotope Biogeochemistry Laboratory. This work was funded by the Penn State Astrobiology Research Center (through the National Astrobiology Institute), NOAA-NURP (UAF 05-0132), the National Science Foundation (MCB-0348492 and OCE-0085549), and the ACS-PRF. The UCLA ion Microprobe is partially supported by a grant from the National Science Foundation Instrumentation and Facilities Program. Also, the ion microprobe work in this paper was supported by a grant from the Moore Foundation. Graduate support (B.T.) for this project was provided by the Penn State Biogeochemical Research Initiative for Education funded by NSF (IGERT) Grant DGE-9972759. Supporting information: Additional Supporting Information may be found in the online version of this article: Fig. S1. An drawing of the in situ incubation device (‘porewater peeper’) used to place Si-wafers and glass slides into seep sediment. There are a series of seven 1-inch-diameter glass slides and seven 1 inch Si-wafers placed so that the surfaces were in contact with a 1 ml internal volume of anoxic 20 g l-1 NaCl solution. The slides and wafers are behind either a 0.2 mm polycarbonate filter (Whatman) or a 12 mm polycarbonate filter. There is also a mesh covering protecting the filters. Insert: photograph of peeper after 14 months in seep sediment. The mesh covering can be seen in each of the holes. Table S1. FISH-SIMS d13C data for microorganisms in PC55 from the Eel River Basin.
Funders:
Funding AgencyGrant Number
Penn State Astrobiology Research CenterUNSPECIFIED
National Oceanic and Atmospheric Administration (NOAA)UAF 05-0132
NSFMCB-0348492
NSFOCE-0085549
American Chemical Society Petroleum Research FundUNSPECIFIED
Gordon and Betty Moore FoundationUNSPECIFIED
NSFDGE-9972759
Issue or Number:9
Record Number:CaltechAUTHORS:20090923-143137088
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20090923-143137088
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:16031
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:06 Oct 2009 19:21
Last Modified:09 Mar 2020 13:18

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