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Human dihydrofolate reductase gene is located in chromosome 5 and is unlinked to the related pseudogenes

Maurer, Barry J. and Barker, P. E. and Masters, Jeffrey N. and Ruddle, Frank H. and Attardi, Giuseppe (1984) Human dihydrofolate reductase gene is located in chromosome 5 and is unlinked to the related pseudogenes. Proceedings of the National Academy of Sciences of the United States of America, 81 (5). pp. 1484-1488. ISSN 0027-8424. PMCID PMC344861. doi:10.1073/pnas.81.5.1484.

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The chromosomal location of the human dihydrofolate reductase (DHFR; EC gene that is amplified in a methotrexate-resistant human cell line has been investigated by screening a large number of human-mouse cell hybrids containing overlapping subsets of human chromosomes. A correlation of genomic blotting data with the chromosome constitution of the individual cell hybrids has allowed the assignment of the human DHFR gene to chromosome 5. This chromosome assignment has been confirmed by the observation of a concomitant loss of the human DHFR gene and of sensitivity to diphtheria toxin, a marker associated with chromosome 5, in two human-mouse cell hybrids selected for resistance to the toxin. Six EcoRI fragments of human DNA containing DHFR pseudogenes or other DHFR-related sequences have been assigned to chromosomes other than chromosome 5.

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Additional Information:© 1984 by the National Academy of Sciences. Communicated by Ray D. Owen, November 16, 1983. We thank Dr. Peter L. Pearson, Instituut voor Anthropogenetica, Leiden, The Netherlands, for making the chromosome 5 probe 1.7 available. We thank Christina Gillies for expert cell culture help and John Hart for isozyme analysis of cell hybrids. P.E.B. is a Postdoctoral Fellow of the Muscular Dystrophy Association. This work was supported in part by National Research Service Award Predoctoral Training Grant T 32 GM-07616 to B.M. and J.N.M. and National Institutes of Health Grants GM-11726 to G.A. and GM-09966 to F.H.R. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
Muscular Dystrophy AssociationUNSPECIFIED
NIH Predoctoral FellowshipT 32 GM-07616
Subject Keywords:human-mouse cell hybrids; DNA transfer hybridization; cDNA; diphtheria toxin sensitivity
Issue or Number:5
PubMed Central ID:PMC344861
Record Number:CaltechAUTHORS:MAUpnas84
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1613
Deposited By: Tony Diaz
Deposited On:07 Feb 2006
Last Modified:08 Nov 2021 19:11

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