Ojala, Deanna and Attardi, Giuseppe (1974) Identification of discrete polyadenylate-containing RNA components transcribed from HeLa cell mitochondrial DNA. Proceedings of the National Academy of Sciences of the United States of America, 71 (2). pp. 563-567. ISSN 0027-8424. https://resolver.caltech.edu/CaltechAUTHORS:OJApnas74
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Abstract
Polyacrylamide gel electrophoresis and sedimentation analysis under denaturing conditions of poly(A)-containing RNA from the polysome region of the sedimentation pattern of a HeLa-cell mitochondrial lysate has revealed the occurrence of a discrete RNA component, which sediments in the native state with a sedimentation constant of about 7 S. From the sedimentation behavior under native and denaturing conditions and the poly(A) content, a molecular weight of about 9 x 10(4) has been estimated for this component. RNA·DNA hybridization experiments have indicated that this component is coded for by the light strand of mitochondrial DNA. Evidence for the occurrence of a poly(A)-containing RNA component sedimenting at about 9 S and coded for by the heavy strand has also been obtained.
| Item Type: | Article | ||||||
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| Additional Information: | Copyright © 1974 by the National Academy of Sciences. Communicated by Jerome Vinograd, October 1, 1973. The help of Mrs. A. Drew and Miss G. Engel is gratefully acknowledged. Poly(dT)-cellulose was a gift of Dr. Joseph Kates and actinomycin D, a gift of Merck, Sharp & Dohme. These investigations were supported by a research grant from the U.S. Public Health Service (GM-11726). | ||||||
| Subject Keywords: | actinomycin D; denaturation; poly(dT)-cellulose; polyacrylamide gel; separated strands; RNA-DNA hybridization | ||||||
| Issue or Number: | 2 | ||||||
| Record Number: | CaltechAUTHORS:OJApnas74 | ||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:OJApnas74 | ||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||
| ID Code: | 1615 | ||||||
| Collection: | CaltechAUTHORS | ||||||
| Deposited By: | Tony Diaz | ||||||
| Deposited On: | 07 Feb 2006 | ||||||
| Last Modified: | 02 Oct 2019 22:45 |
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