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Cell-selective metabolic labeling of proteins

Ngo, John T. and Champion, Julie A. and Mahdavi, Alborz and Tanrikulu, I. Caglar and Beatty, Kimberly E. and Connor, Rebecca E. and Yoo, Tae Hyeon and Dieterich, Daniela C. and Schuman, Erin M. and Tirrell, David A. (2009) Cell-selective metabolic labeling of proteins. Nature Chemical Biology, 5 (10). pp. 715-717. ISSN 1552-4450. PMCID PMC3176724.

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Metabolic labeling of proteins with the methionine surrogate azidonorleucine can be targeted exclusively to specified cells through expression of a mutant methionyl-tRNA synthetase (MetRS). In complex cellular mixtures, proteins made in cells that express the mutant synthetase can be tagged with affinity reagents (for detection or enrichment) or fluorescent dyes (for imaging). Proteins made in cells that do not express the mutant synthetase are neither labeled nor detected.

Item Type:Article
Related URLs:
URLURL TypeDescription ReadCube access CentralArticle
Mahdavi, Alborz0000-0002-8790-8112
Beatty, Kimberly E.0000-0002-2303-8779
Yoo, Tae Hyeon0000-0003-1448-3165
Schuman, Erin M.0000-0002-7053-1005
Tirrell, David A.0000-0003-3175-4596
Additional Information:© 2009 Nature Publishing Group. Received 28 October 2008; Accepted 28 May 2009; Published online 9 August 2009. We gratefully acknowledge the US National Institutes of Health (R21 DA020589 and R01 GM62523) and the US Army Research Office Institute for Collaborative Biotechnologies for support of this work. We thank K. Boulware for assistance in generating the three-dimensional movie of infected macrophages. E.M.S. is an investigator of the Howard Hughes Medical Institute. Note: Supplementary information and chemical compound information is available on the Nature Chemical Biology website.
Funding AgencyGrant Number
NIHR21 DA020589
NIHR01 GM62523
Army Research Office (ARO)UNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:10
PubMed Central ID:PMC3176724
Record Number:CaltechAUTHORS:20091013-093448078
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:16297
Deposited By: George Porter
Deposited On:21 Oct 2009 21:26
Last Modified:28 Feb 2020 00:14

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