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Gal4 turnover and transcription activation

Collins, Galen A. and Lipford, J. Russell and Deshaies, Raymond J. and Tansey, William P. (2009) Gal4 turnover and transcription activation. Nature, 461 (7265). E7-E8. ISSN 0028-0836. PMCID PMC3072683. https://resolver.caltech.edu/CaltechAUTHORS:20091022-111502275

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Abstract

Growing evidence supports the notion that proteasome-mediated destruction of transcriptional activators can be intimately coupled to their function. Recently, Nalley et al. challenged this view by reporting that the prototypical yeast activator Gal4 does not dynamically associate with chromatin, but rather 'locks in' to stable promoter complexes that are resistant to competition. Here we present evidence that the assay used to reach this conclusion is unsuitable, and that promoter-bound, active Gal4 is indeed susceptible to competition in vivo. Our data challenge the key evidence that Nalley et al. used to reach their conclusion, and indicate that Gal4 functions in vivo within the context of dynamic promoter complexes.


Item Type:Article
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http://dx.doi.org/10.1038/nature08406DOIArticle
http://www.nature.com/nature/journal/v461/n7265/full/nature08406.htmlPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3072683/PubMed CentralArticle
http://rdcu.be/c2EpPublisherFree ReadCube access
ORCID:
AuthorORCID
Deshaies, Raymond J.0000-0002-3671-9354
Additional Information:© 2009 Nature Publishing Group. Received 20 February 2009; Accepted 13 August 2009.
Issue or Number:7265
PubMed Central ID:PMC3072683
Record Number:CaltechAUTHORS:20091022-111502275
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20091022-111502275
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:16445
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:22 Oct 2009 18:52
Last Modified:03 Oct 2019 01:12

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