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The application of on-chip optofluidic microscopy for imaging Giardia lamblia trophozoites and cysts

Lee, Lap Man and Cui, Xiquan and Yang, Changhuei (2009) The application of on-chip optofluidic microscopy for imaging Giardia lamblia trophozoites and cysts. Biomedical Microdevices, 11 (5). pp. 951-958. ISSN 1387-2176 . PMCID PMC2888668. http://resolver.caltech.edu/CaltechAUTHORS:20091029-142950975

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Abstract

The optofluidic microscope (OFM) is a lensless, low-cost and highly compact on-chip device that can enable high-resolution microscopy imaging. The OFM performs imaging by flowing/scanning the target objects across a slanted hole array; by measuring the time-varying light transmission changes through the holes, we can then render images of the target objects at a resolution that is comparable to the holes’ size. This paper reports the adaptation of the OFM for imaging Giardia lamblia trophozoites and cysts, a disease-causing parasite species that is commonly found in poor-quality water sources. We also describe our study of the impact of pressure-based flow and DC electrokinetic-based flow in controlling the flow motion of Giardia cysts—rotation-free translation of the parasite is important for good OFM image acquisition. Finally, we report the successful microscopy imaging of both Giardia trophozoites and cysts with an OFM that has a focal plane resolution of 0.8 microns.


Item Type:Article
Related URLs:
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http://dx.doi.org/10.1007/s10544-009-9312-xDOIArticle
http://www.springerlink.com/content/pn340633732l0401/PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2888668/PubMed CentralArticle
http://rdcu.be/p3cVPublisherFree ReadCube access
Additional Information:© Springer Science + Business Media, LLC 2009. Published online 14 April 2009. The authors appreciate the generous help and discussions from Dr. Xin Heng, Guoan Zheng, Sean Pang and Dr. Jigang Wu. This work is supported by DARPA Center for Optofluidic Integration (California Institute of Technology), NIH R21EB008867-01, and Coulter Foundation Early Career Award. Lap Man Lee is thankful for the financial support from the Croucher Foundation Scholarship (Hong Kong).
Group:Kavli Nanoscience Institute
Funders:
Funding AgencyGrant Number
NIHR21 EB008867-01
Wallace Coulter FoundationUNSPECIFIED
Croucher FoundationUNSPECIFIED
Subject Keywords:Optofluidic microscopy; Microfluidics; On-chip imaging; Water quality monitoring; DC electrokinetics
Issue or Number:5
PubMed Central ID:PMC2888668
Record Number:CaltechAUTHORS:20091029-142950975
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20091029-142950975
Official Citation:Lee, L.M., Cui, X. & Yang, C. Biomed Microdevices (2009) 11: 951. doi:10.1007/s10544-009-9312-x
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:16523
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:02 Nov 2009 19:44
Last Modified:13 Mar 2017 21:28

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