CaltechAUTHORS
  A Caltech Library Service

The cis-regulatory system of the tbrain gene: Alternative use of multiple modules to promote skeletogenic expression in the sea urchin embryo

Wahl, Mary E. and Hahn, Julie and Gora, Kasia and Davidson, Eric H. and Oliveri, Paola (2009) The cis-regulatory system of the tbrain gene: Alternative use of multiple modules to promote skeletogenic expression in the sea urchin embryo. Developmental Biology, 335 (2). pp. 428-441. ISSN 0012-1606. PMCID PMC2784196. https://resolver.caltech.edu/CaltechAUTHORS:20091123-113132667

[img] PDF - Accepted Version
See Usage Policy.

4022Kb
[img]
Preview
PDF (Supplementary Figure 1) - Supplemental Material
See Usage Policy.

529Kb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20091123-113132667

Abstract

The genomic cis-regulatory systems controlling regulatory gene expression usually include multiple modules. The regulatory output of such systems at any given time depends on which module is directing the function of the basal transcription apparatus, and ultimately on the transcription factor inputs into that module. Here we examine regulation of the Strongylocentrotus purpuratus tbrain gene, a required activator of the skeletogenic specification state in the lineage descendant from the embryo micromeres. Alternate cis-regulatory modules were found to convey skeletogenic expression in reporter constructs. To determine their relative developmental functions in context, we made use of recombineered BAC constructs containing a GFP reporter and of derivatives from which specific modules had been deleted. The outputs of the various constructs were observed spatially by GFP fluorescence and quantitatively over time by QPCR. In the context of the complete genomic locus, early skeletogenic expression is controlled by an intron enhancer plus a proximal region containing a HesC site as predicted from network analysis. From ingression onward, however, a dedicated distal module utilizing positive Ets1/2 inputs contributes to definitive expression in the skeletogenic mesenchyme. This module also mediates a newly discovered negative Erg input which excludes non-skeletogenic mesodermal expression.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.ydbio.2009.08.005DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160609011142PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2784196/PubMed CentralArticle
Additional Information:© 2009 Elsevier Inc. Received 27 March 2009; revised 24 July 2009; accepted 3 August 2009. Available online 11 August 2009. We would like to thank Prof. Andrew Murray and anonymous reviewers for critical reading and helpful suggestions. Research was supported by the Caltech SURF program, the Camilla Chandler Frost Fellowship, the U.S. Department of Defense NDSEG Fellowship Program, and NIH grants HD037105 and GM075089.
Funders:
Funding AgencyGrant Number
Caltech SURF programUNSPECIFIED
Camilla Chandler Frost FellowshipUNSPECIFIED
U. D. Department of Defense NDSEG FellowshipUNSPECIFIED
NIHHD037105
NIHGM075089
Subject Keywords:tbrain gene; Gene regulatory network; cis-Regulatory analysis; Skeletogenic micromere lineage; Recombinant BAC
Issue or Number:2
PubMed Central ID:PMC2784196
Record Number:CaltechAUTHORS:20091123-113132667
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20091123-113132667
Official Citation:Mary E. Wahl, Julie Hahn, Kasia Gora, Eric H. Davidson, Paola Oliveri, The cis-regulatory system of the tbrain gene: Alternative use of multiple modules to promote skeletogenic expression in the sea urchin embryo, Developmental Biology, Volume 335, Issue 2, 15 November 2009, Pages 428-441, ISSN 0012-1606, http://dx.doi.org/10.1016/j.ydbio.2009.08.005. (http://www.sciencedirect.com/science/article/pii/S0012160609011142)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:16782
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:24 Nov 2009 22:13
Last Modified:03 Oct 2019 01:17

Repository Staff Only: item control page