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Mechanism of active targeting in solid tumors with transferrin-containing gold nanoparticles

Choi, Chung Hang J. and Alabi, Christopher A. and Webster, Paul and Davis, Mark E. (2010) Mechanism of active targeting in solid tumors with transferrin-containing gold nanoparticles. Proceedings of the National Academy of Sciences of the United States of America, 107 (3). pp. 1235-1240. ISSN 0027-8424. PMCID PMC2824286. doi:10.1073/pnas.0914140107.

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PEGylated gold nanoparticles are decorated with various amounts of human transferrin (Tf) to give a series of Tf-targeted particles with near-constant size and electrokinetic potential. The effects of Tf content on nanoparticle tumor targeting were investigated in mice bearing s.c. Neuro2A tumors. Quantitative biodistributions of the nanoparticles 24 h after i.v. tail-vein injections show that the nanoparticle accumulations in the tumors and other organs are independent of Tf. However, the nanoparticle localizations within a particular organ are influenced by the Tf content. In tumor tissue, the content of targeting ligands significantly influences the number of nanoparticles localized within the cancer cells. In liver tissue, high Tf content leads to small amounts of the nanoparticles residing in hepatocytes, whereas most nanoparticles remain in nonparenchymal cells. These results suggest that targeted nanoparticles can provide greater intracellular delivery of therapeutic agents to the cancer cells within solid tumors than their nontargeted analogs.

Item Type:Article
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URLURL TypeDescription DOIArticle CentralArticle Information
Alabi, Christopher A.0000-0003-2654-018X
Davis, Mark E.0000-0001-8294-1477
Additional Information:© 2010 by the National Academy of Sciences. Freely available online through the PNAS open access option. Contributed by Mark E. Davis, December 8, 2009 (sent for review November 24, 2009). Published online before print December 29, 2009. We thank Debbie Guerrero, Siva Wu, Jennifer Bower (House Ear Institute), Carol Garland, and Nathan Dalleska (California Institute of Technology) for advice on histology, transmission electron microscopy, and ICP-MS. This work was supported by National Cancer Institute Grant CA 119347 and National Institutes of Health Grant R01 EB004657. Author contributions: C.H.J.C. and M.E.D. designed research; C.H.J.C. and C.A.A. performed research; C.A.A., P.W., and M.E.D. analyzed data; and C.H.J.C. and M.E.D. wrote the paper.
Funding AgencyGrant Number
NIHCA 119347
NIHR01 EB004657
National Cancer InstituteUNSPECIFIED
Subject Keywords:in vivo distribution; intracellular localization; ligand content; targeted delivery
Issue or Number:3
PubMed Central ID:PMC2824286
Record Number:CaltechAUTHORS:20100217-134833200
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Official Citation:Chung Hang J. Choi, Christopher A. Alabi, Paul Webster, and Mark E. Davis Mechanism of active targeting in solid tumors with transferrin-containing gold nanoparticlesPNAS 2010 107:1235-1240; published online before print December 29, 2009, doi:10.1073/pnas.0914140107
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:17508
Deposited By: Tony Diaz
Deposited On:19 Feb 2010 17:53
Last Modified:08 Nov 2021 23:36

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